Abstract
The Bacillus sphaericus binary toxin is expressed during the early stages of sporulation and is composed of two separately encoded polypeptides: BinA (41.9 kDa) and BinB (51.4 kDa). The binary toxin forms microcrystalline inclusions inside the mother cell that, once ingested, are solubilized in the alkaline pH of the larval gut. B. sphaericus cultures were grown to complete sporulation, harvested and washed. The binary toxin was solubilized with 50 mM NaOH, purified using column chromatography and crystallized. Crystals were grown using the sitting-drop vapor-diffusion method in a glycine-buffered solution containing 0.5 M NaCl and 19.5% PEG 4000 as the precipitant. Native data were collected to a resolution of 2.7 Å.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 952-953 |
| Number of pages | 2 |
| Journal | Acta Crystallographica Section D: Biological Crystallography |
| Volume | 60 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2004 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biophysics
- Condensed Matter Physics
- Structural Biology
Cite this
Crystallization of the mosquito-larvicidal binary toxin produced by Bacillus sphaericus. / Smith, Alex W.; Camara-Artigas, Ana; Allen, James.
In: Acta Crystallographica Section D: Biological Crystallography, Vol. 60, No. 5, 05.2004, p. 952-953.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Crystallization of the mosquito-larvicidal binary toxin produced by Bacillus sphaericus
AU - Smith, Alex W.
AU - Camara-Artigas, Ana
AU - Allen, James
PY - 2004/5
Y1 - 2004/5
N2 - The Bacillus sphaericus binary toxin is expressed during the early stages of sporulation and is composed of two separately encoded polypeptides: BinA (41.9 kDa) and BinB (51.4 kDa). The binary toxin forms microcrystalline inclusions inside the mother cell that, once ingested, are solubilized in the alkaline pH of the larval gut. B. sphaericus cultures were grown to complete sporulation, harvested and washed. The binary toxin was solubilized with 50 mM NaOH, purified using column chromatography and crystallized. Crystals were grown using the sitting-drop vapor-diffusion method in a glycine-buffered solution containing 0.5 M NaCl and 19.5% PEG 4000 as the precipitant. Native data were collected to a resolution of 2.7 Å.
AB - The Bacillus sphaericus binary toxin is expressed during the early stages of sporulation and is composed of two separately encoded polypeptides: BinA (41.9 kDa) and BinB (51.4 kDa). The binary toxin forms microcrystalline inclusions inside the mother cell that, once ingested, are solubilized in the alkaline pH of the larval gut. B. sphaericus cultures were grown to complete sporulation, harvested and washed. The binary toxin was solubilized with 50 mM NaOH, purified using column chromatography and crystallized. Crystals were grown using the sitting-drop vapor-diffusion method in a glycine-buffered solution containing 0.5 M NaCl and 19.5% PEG 4000 as the precipitant. Native data were collected to a resolution of 2.7 Å.
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UR - http://www.scopus.com/inward/citedby.url?scp=16644388981&partnerID=8YFLogxK
U2 - 10.1107/S0907444904006535
DO - 10.1107/S0907444904006535
M3 - Article
C2 - 15103150
AN - SCOPUS:16644388981
VL - 60
SP - 952
EP - 953
JO - Acta Crystallographica Section D: Structural Biology
JF - Acta Crystallographica Section D: Structural Biology
SN - 0907-4449
IS - 5
ER -