TY - JOUR
T1 - Crystallization of intact and subunit L-deficient monomers from synechocystis PCC 6803 photosystem I
AU - Jekow, Petra
AU - Schubert, Wolf Dieter
AU - Fromme, Petra
AU - Kruip, Jochen
AU - Chitnis, Parag R.
AU - Rögner, Matthias
AU - Saenger, Wolfram
PY - 1996
Y1 - 1996
N2 - Photosystem I monomers from wildtype cells of Synechocystis PCC 6803 and from a mutant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 Å, c = 525 Å, α = β = 90°, y = 120°, the space group is P61 or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement of crystal structure data.
AB - Photosystem I monomers from wildtype cells of Synechocystis PCC 6803 and from a mutant deficient in the psaL gene were crystallized. PsaL encodes for the hydrophobic subunit L, which has been proposed to constitute the trimerization domain in the PS I trimer. The absence of subunit L facilitated crystallization of the PS I monomer. The unit cell dimensions and the space group for the crystals from this preparation could be determined to be a = b = 132 Å, c = 525 Å, α = β = 90°, y = 120°, the space group is P61 or P65. The results show the potential of using specifically designed deletion mutants of an integral membrane protein for the systematic improvement of crystal structure data.
KW - Crystallization
KW - Mesophilic Cyanobacterium
KW - PS I Monomers
KW - Photosystem I
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U2 - 10.1515/znc-1996-3-410
DO - 10.1515/znc-1996-3-410
M3 - Article
AN - SCOPUS:11344267612
VL - 51
SP - 195
EP - 199
JO - Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie
JF - Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie
SN - 0939-5075
IS - 3-4
ER -