Crystallization and preliminary X-ray diffraction analysis of cytochrome c2 from Rhodobacter sphaeroides

J. P. Allen

doi:10.1016/0022-2836(88)90592-X

Crystallization and preliminary X-ray diffraction analysis of cytochrome c₂ from Rhodobacter sphaeroides

J. P. Allen

Research output: Contribution to journal › Letter › peer-review

3 Scopus citations

Abstract

Cytochrome c₂ from Rhodobacter sphaeroides, which serves as the secondary donor in bacterial photosynthesis, has been crystallized from ammonium sulfate solutions at pH 7.0 by vapor diffusion. The crystals are well formed with clearly discernable facets. Their space group is P6₅22 (or its enantiomorph P6₁22) with unit cell dimensions a = b = 64.5 A ̊ and c = 163.0 A ̊, and two proteins per asymmetric unit. The crystals diffract to at least 2.7 Å and are stable during X-ray exposure for several days.

Original language	English (US)
Pages (from-to)	495-496
Number of pages	2
Journal	Journal of molecular biology
Volume	204
Issue number	2
DOIs	https://doi.org/10.1016/0022-2836(88)90592-X
State	Published - Nov 20 1988
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(88)90592-X

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title = "Crystallization and preliminary X-ray diffraction analysis of cytochrome c2 from Rhodobacter sphaeroides",

abstract = "Cytochrome c2 from Rhodobacter sphaeroides, which serves as the secondary donor in bacterial photosynthesis, has been crystallized from ammonium sulfate solutions at pH 7.0 by vapor diffusion. The crystals are well formed with clearly discernable facets. Their space group is P6522 (or its enantiomorph P6122) with unit cell dimensions a = b = 64.5 A ̊ and c = 163.0 A ̊, and two proteins per asymmetric unit. The crystals diffract to at least 2.7 {\AA} and are stable during X-ray exposure for several days.",

author = "Allen, {J. P.}",

note = "Funding Information: The author is grateful to R. Bartsch for providing the preparation of cytochrome cl, and to D. C. Rees and G. Feher for use of laboratory facilities. This work was supported by the National Institutes of Health grant DK36053 and GM13191.",

year = "1988",

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doi = "10.1016/0022-2836(88)90592-X",

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pages = "495--496",

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TY - JOUR

T1 - Crystallization and preliminary X-ray diffraction analysis of cytochrome c2 from Rhodobacter sphaeroides

AU - Allen, J. P.

N1 - Funding Information: The author is grateful to R. Bartsch for providing the preparation of cytochrome cl, and to D. C. Rees and G. Feher for use of laboratory facilities. This work was supported by the National Institutes of Health grant DK36053 and GM13191.

PY - 1988/11/20

Y1 - 1988/11/20

N2 - Cytochrome c2 from Rhodobacter sphaeroides, which serves as the secondary donor in bacterial photosynthesis, has been crystallized from ammonium sulfate solutions at pH 7.0 by vapor diffusion. The crystals are well formed with clearly discernable facets. Their space group is P6522 (or its enantiomorph P6122) with unit cell dimensions a = b = 64.5 A ̊ and c = 163.0 A ̊, and two proteins per asymmetric unit. The crystals diffract to at least 2.7 Å and are stable during X-ray exposure for several days.

AB - Cytochrome c2 from Rhodobacter sphaeroides, which serves as the secondary donor in bacterial photosynthesis, has been crystallized from ammonium sulfate solutions at pH 7.0 by vapor diffusion. The crystals are well formed with clearly discernable facets. Their space group is P6522 (or its enantiomorph P6122) with unit cell dimensions a = b = 64.5 A ̊ and c = 163.0 A ̊, and two proteins per asymmetric unit. The crystals diffract to at least 2.7 Å and are stable during X-ray exposure for several days.

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DO - 10.1016/0022-2836(88)90592-X

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JO - Journal of molecular biology

JF - Journal of molecular biology

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ER -

Crystallization and preliminary X-ray diffraction analysis of cytochrome c₂ from Rhodobacter sphaeroides

Abstract

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