Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes

Jose S. Santos, Guillermo A. Asmar-Rovira, Gye Won Han, Wei Liu, Ruhma Syeda, Vadim Cherezov, Kent A. Baker, Raymond C. Stevens, Mauricio Montal

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Voltage-gated K+ channels underlie the electrical excitability of cells. Each subunit of the functional tetramer consists of the tandem fusion of two modules, an N-terminal voltage-sensor and a C-terminal pore. To investigate how sensor coupling to the pore generates voltage-dependent channel opening, we solved the crystal structure and characterized the function of a voltage-gated K+ channel pore in a lipid membrane. The structure of a functional channel in a membrane environment at 3.1Åresolution establishes an unprecedented connection between channel structure and function. The structure is unique in delineating an ion-occupied ready to conduct selectivity filter, a confined aqueous cavity, and a closed activation gate, embodying a dynamic entity trapped in an unstable closed state.

Original languageEnglish (US)
Pages (from-to)43063-43070
Number of pages8
JournalJournal of Biological Chemistry
Volume287
Issue number51
DOIs
StatePublished - Dec 14 2012
Externally publishedYes

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Voltage-Gated Potassium Channels
Membrane Lipids
Crystal structure
Sensors
Electric potential
Fusion reactions
Chemical activation
Ions
Membranes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Santos, J. S., Asmar-Rovira, G. A., Han, G. W., Liu, W., Syeda, R., Cherezov, V., ... Montal, M. (2012). Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes. Journal of Biological Chemistry, 287(51), 43063-43070. https://doi.org/10.1074/jbc.M112.415091

Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes. / Santos, Jose S.; Asmar-Rovira, Guillermo A.; Han, Gye Won; Liu, Wei; Syeda, Ruhma; Cherezov, Vadim; Baker, Kent A.; Stevens, Raymond C.; Montal, Mauricio.

In: Journal of Biological Chemistry, Vol. 287, No. 51, 14.12.2012, p. 43063-43070.

Research output: Contribution to journalArticle

Santos, JS, Asmar-Rovira, GA, Han, GW, Liu, W, Syeda, R, Cherezov, V, Baker, KA, Stevens, RC & Montal, M 2012, 'Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes', Journal of Biological Chemistry, vol. 287, no. 51, pp. 43063-43070. https://doi.org/10.1074/jbc.M112.415091
Santos, Jose S. ; Asmar-Rovira, Guillermo A. ; Han, Gye Won ; Liu, Wei ; Syeda, Ruhma ; Cherezov, Vadim ; Baker, Kent A. ; Stevens, Raymond C. ; Montal, Mauricio. / Crystal structure of a voltage-gated K+ channel pore module in a closed state in lipid membranes. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 51. pp. 43063-43070.
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