Crystal structure of a conserved protease that binds DNA: The bleomycin hydrolase, Gal6

Leemor Joshua-Tor, H. Eric Xu, Stephen Johnston, Douglas C. Rees

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Bleomycin hydrolase is a cysteine protease that hydrolyzes the anticancer drug bleomycin. The homolog in yeast, Gal6, has recently been identified and found to bind DNA and to act as a repressor in the Gal4 regulatory system. The crystal structure of Gal6 at 2.2 Å resolution reveals a hexameric structure with a prominent central channel. The papain-like active sites are situated within the central channel, in a manner resembling the organization of active sites in the proteasome. The Gal6 channel is lined with 60 lysine residues from the six subunits, suggesting a role in DNA binding. The carboxyl-terminal arm of Gal6 extends into the active site cleft and may serve a regulatory function. Rather than each residing in distinct, separable domains, the protease and DNA-binding activities appear structurally intertwined in the hexamer, implying a coupling of these two activities.

Original languageEnglish (US)
Pages (from-to)945-950
Number of pages6
JournalScience
Volume269
Issue number5226
StatePublished - Aug 18 1995
Externally publishedYes

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Catalytic Domain
Peptide Hydrolases
DNA
Papain
Cysteine Proteases
Bleomycin
Proteasome Endopeptidase Complex
Lysine
Yeasts
Pharmaceutical Preparations
bleomycin hydrolase

ASJC Scopus subject areas

  • General

Cite this

Joshua-Tor, L., Xu, H. E., Johnston, S., & Rees, D. C. (1995). Crystal structure of a conserved protease that binds DNA: The bleomycin hydrolase, Gal6. Science, 269(5226), 945-950.

Crystal structure of a conserved protease that binds DNA : The bleomycin hydrolase, Gal6. / Joshua-Tor, Leemor; Xu, H. Eric; Johnston, Stephen; Rees, Douglas C.

In: Science, Vol. 269, No. 5226, 18.08.1995, p. 945-950.

Research output: Contribution to journalArticle

Joshua-Tor, L, Xu, HE, Johnston, S & Rees, DC 1995, 'Crystal structure of a conserved protease that binds DNA: The bleomycin hydrolase, Gal6', Science, vol. 269, no. 5226, pp. 945-950.
Joshua-Tor, Leemor ; Xu, H. Eric ; Johnston, Stephen ; Rees, Douglas C. / Crystal structure of a conserved protease that binds DNA : The bleomycin hydrolase, Gal6. In: Science. 1995 ; Vol. 269, No. 5226. pp. 945-950.
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