Critical evaluation of membrane supports for use in quantitative hybridizations

Lutgarde Raskin, William C. Capman, Matthew D. Kane, Bruce E. Rittmann, David A. Stahl

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher and Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.

Original languageEnglish (US)
Pages (from-to)300-303
Number of pages4
JournalApplied and environmental microbiology
Volume62
Issue number1
DOIs
StatePublished - 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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