TY - JOUR
T1 - Critical evaluation of membrane supports for use in quantitative hybridizations
AU - Raskin, Lutgarde
AU - Capman, William C.
AU - Kane, Matthew D.
AU - Rittmann, Bruce E.
AU - Stahl, David A.
PY - 1996
Y1 - 1996
N2 - The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher and Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.
AB - The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher and Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.
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U2 - 10.1128/aem.62.1.300-303.1996
DO - 10.1128/aem.62.1.300-303.1996
M3 - Article
C2 - 16535220
AN - SCOPUS:0030038428
SN - 0099-2240
VL - 62
SP - 300
EP - 303
JO - Applied and environmental microbiology
JF - Applied and environmental microbiology
IS - 1
ER -