Critical evaluation of membrane supports for use in quantitative hybridizations

Lutgarde Raskin, William C. Capman, Matthew D. Kane, Bruce Rittmann, David A. Stahl

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher and Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of detectability provided by the Magna Charge and Immobilon-N membranes were 20 to 50 times better than those obtained with the MagnaGraph, Magna, and Nytran membranes. The variability of the signal response for individual membranes ranged from 10 to 50%, with the Magna Charge and Immobilon-N membranes demonstrating the lowest variability.

Original languageEnglish (US)
Pages (from-to)300-303
Number of pages4
JournalApplied and Environmental Microbiology
Volume62
Issue number1
StatePublished - 1996
Externally publishedYes

Fingerprint

hybridization
membrane
Membranes
oligonucleotide probes
corporations
nucleic acids
ribosomal RNA
Oligonucleotide Probes
nucleic acid
Immobilization
immobilization
Nucleic Acids
probe
evaluation
Immobilon

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Science(all)
  • Microbiology

Cite this

Critical evaluation of membrane supports for use in quantitative hybridizations. / Raskin, Lutgarde; Capman, William C.; Kane, Matthew D.; Rittmann, Bruce; Stahl, David A.

In: Applied and Environmental Microbiology, Vol. 62, No. 1, 1996, p. 300-303.

Research output: Contribution to journalArticle

Raskin, Lutgarde ; Capman, William C. ; Kane, Matthew D. ; Rittmann, Bruce ; Stahl, David A. / Critical evaluation of membrane supports for use in quantitative hybridizations. In: Applied and Environmental Microbiology. 1996 ; Vol. 62, No. 1. pp. 300-303.
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