CRISPR transcriptional repression devices and layered circuits in mammalian cells

Samira Kiani, Jacob Beal, Mohammad Ebrahimkhani, Jin Huh, Richard N. Hall, Zhen Xie, Yinqing Li, Ron Weiss

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.

Original languageEnglish (US)
Pages (from-to)723-726
Number of pages4
JournalNature Methods
Volume11
Issue number7
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Guide RNA
Cells
Equipment and Supplies
Networks (circuits)
Libraries

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

CRISPR transcriptional repression devices and layered circuits in mammalian cells. / Kiani, Samira; Beal, Jacob; Ebrahimkhani, Mohammad; Huh, Jin; Hall, Richard N.; Xie, Zhen; Li, Yinqing; Weiss, Ron.

In: Nature Methods, Vol. 11, No. 7, 2014, p. 723-726.

Research output: Contribution to journalArticle

Kiani, Samira ; Beal, Jacob ; Ebrahimkhani, Mohammad ; Huh, Jin ; Hall, Richard N. ; Xie, Zhen ; Li, Yinqing ; Weiss, Ron. / CRISPR transcriptional repression devices and layered circuits in mammalian cells. In: Nature Methods. 2014 ; Vol. 11, No. 7. pp. 723-726.
@article{48e2d00d05644a28ad1b0f0ac7d043da,
title = "CRISPR transcriptional repression devices and layered circuits in mammalian cells",
abstract = "A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.",
author = "Samira Kiani and Jacob Beal and Mohammad Ebrahimkhani and Jin Huh and Hall, {Richard N.} and Zhen Xie and Yinqing Li and Ron Weiss",
year = "2014",
doi = "10.1038/nmeth.2969",
language = "English (US)",
volume = "11",
pages = "723--726",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "7",

}

TY - JOUR

T1 - CRISPR transcriptional repression devices and layered circuits in mammalian cells

AU - Kiani, Samira

AU - Beal, Jacob

AU - Ebrahimkhani, Mohammad

AU - Huh, Jin

AU - Hall, Richard N.

AU - Xie, Zhen

AU - Li, Yinqing

AU - Weiss, Ron

PY - 2014

Y1 - 2014

N2 - A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.

AB - A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.

UR - http://www.scopus.com/inward/record.url?scp=84903618690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903618690&partnerID=8YFLogxK

U2 - 10.1038/nmeth.2969

DO - 10.1038/nmeth.2969

M3 - Article

VL - 11

SP - 723

EP - 726

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 7

ER -