CRISPR transcriptional repression devices and layered circuits in mammalian cells

Samira Kiani, Jacob Beal, Mohammad R. Ebrahimkhani, Jin Huh, Richard N. Hall, Zhen Xie, Yinqing Li, Ron Weiss

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.

Original languageEnglish (US)
Pages (from-to)723-726
Number of pages4
JournalNature Methods
Volume11
Issue number7
DOIs
StatePublished - Jul 2014

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kiani, S., Beal, J., Ebrahimkhani, M. R., Huh, J., Hall, R. N., Xie, Z., Li, Y., & Weiss, R. (2014). CRISPR transcriptional repression devices and layered circuits in mammalian cells. Nature Methods, 11(7), 723-726. https://doi.org/10.1038/nmeth.2969