Abstract

Background: Biocatalysts provide an economical and energy-efficient alternative to traditional chemical manufacturing processes. For processes where biocatalysts currently do not exist or existing protein catalysts function poorly, there is a tremendous need to discover new protein catalysts that function in industrial settings. The protein engineering community has traditionally relied on cell-based techniques in 96-well format to evolve new catalysts or improve existing enzymes. Objective: This review examines recent progress made in many display technologies, providing powerful alternatives for generating novel enzymes with altered specificity or altogether new types of function. Methods: Library creation methods and display technologies that are commonly used in conjunction with enzyme evolution are discussed. Conclusion: We conclude with an expert opinion on future trans-disciplinary approaches that combine directed evolution with computational design as novel platforms for rapidly discovering new types of catalytic function.

Original languageEnglish (US)
Pages (from-to)1087-1098
Number of pages12
JournalExpert Opinion on Biological Therapy
Volume8
Issue number8
DOIs
StatePublished - Aug 2008

Fingerprint

Industrial applications
Enzymes
Proteins
Catalysts
Chemical Phenomena
Display devices
Technology
Protein Engineering
Expert Testimony
Libraries

Keywords

  • Directed evolution
  • Library diversity
  • Protein biocatalyst

ASJC Scopus subject areas

  • Pharmacology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Immunology

Cite this

Creating protein biocatalysts as tools for future industrial applications. / Chaput, John C.; Woodbury, Neal; Stearns, Linda A.; Williams, Berea A R.

In: Expert Opinion on Biological Therapy, Vol. 8, No. 8, 08.2008, p. 1087-1098.

Research output: Contribution to journalArticle

Chaput, John C. ; Woodbury, Neal ; Stearns, Linda A. ; Williams, Berea A R. / Creating protein biocatalysts as tools for future industrial applications. In: Expert Opinion on Biological Therapy. 2008 ; Vol. 8, No. 8. pp. 1087-1098.
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