Cowpox virus expresses a novel ankyrin repeat NF-κB inhibitor that controls inflammatory cell influx into virus-infected tissues and is critical for virus pathogenesis

Mohamed Ragaa Mohamed, Masmudur Rahman, Amanda Rice, Richard W. Moyer, Steven J. Werden, Douglas McFadden

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Many pathogenic orthopoxviruses like variola virus, monkeypox virus, and cowpox virus (CPXV), but not vaccinia virus, encode a unique family of ankyrin (ANK) repeat-containing proteins that interact directly with NF-κB1/p105 and inhibit the NF-κB signaling pathway. Here, we present the in vitro and in vivo characterization of the targeted gene knockout of this novel NF-κB inhibitor in CPXV. Our results demonstrate that the vCpx-006KO uniquely induces a variety of NF-κB-controlled proinflammatory cytokines from infected myeloid cells, accompanied by a rapid phosphorylation of the IκB kinase complex and subsequent degradation of the NF-κB cellular inhibitors IκBα and NF-κB1/p105. Moreover, the vCpx-006KO virus was attenuated for virulence in mice and induced a significantly elevated cellular inflammatory process at tissue sites of virus replication in the lung. These results indicate that members of this ANK repeat family are utilized specifically by pathogenic orthopoxviruses to repress the NF-κB signaling pathway at tissue sites of virus replication in situ.

Original languageEnglish (US)
Pages (from-to)9223-9236
Number of pages14
JournalJournal of Virology
Volume83
Issue number18
DOIs
StatePublished - Sep 1 2009
Externally publishedYes

Fingerprint

Cowpox virus
Orthopoxvirus
Ankyrin Repeat
ankyrins
Virus Replication
virus replication
Monkeypox virus
Variola virus
pathogenesis
Viruses
viruses
Vaccinia virus
Gene Knockout Techniques
gene targeting
Myeloid Cells
Virulence
phosphorylation
phosphotransferases (kinases)
cytokines
Phosphotransferases

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Cowpox virus expresses a novel ankyrin repeat NF-κB inhibitor that controls inflammatory cell influx into virus-infected tissues and is critical for virus pathogenesis. / Mohamed, Mohamed Ragaa; Rahman, Masmudur; Rice, Amanda; Moyer, Richard W.; Werden, Steven J.; McFadden, Douglas.

In: Journal of Virology, Vol. 83, No. 18, 01.09.2009, p. 9223-9236.

Research output: Contribution to journalArticle

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