Abstract
Two-dimensional protein crystallization on lipid monolayers at a quiescent air/water interface is now a well-established process, but it only operates under a very restricted set of conditions and on a very slow time scale. We have recently been able to significantly extend the conditions under which the proteins will crystallize as well as speed up the process by subjecting the interface to a shearing flow. Here, we investigate the two-way coupling between a protein-laden film and the bulk flow that provides the interfacial shear. This flow in a stationary open cylinder is driven by the constant rotation of the floor. Using the Boussinesq-Scriven surface model for a Newtonian interface coupled to the Navier-Stokes equations for the bulk flow, we find that the surface shear viscosity of protein-laden films under most conditions is small or negligible. This is the case for films subjected to constant shearing flow, regardless of the duration of the flow. However, when the film is intermittently sheared, significant surface shear viscosity is evident. In these cases, the surface shear viscosity is not uniform across the film.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-86 |
| Number of pages | 8 |
| Journal | Journal of Colloid and Interface Science |
| Volume | 322 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1 2008 |
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Keywords
- Surface shear viscosity
- Two-dimensional protein crystallization
ASJC Scopus subject areas
- Colloid and Surface Chemistry
- Physical and Theoretical Chemistry
- Surfaces and Interfaces
Cite this
Coupling between protein-laden films and a shearing bulk flow. / Azadani, A. N.; Lopez, Juan; Hirsa, A. H.
In: Journal of Colloid and Interface Science, Vol. 322, No. 1, 01.06.2008, p. 79-86.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Coupling between protein-laden films and a shearing bulk flow
AU - Azadani, A. N.
AU - Lopez, Juan
AU - Hirsa, A. H.
PY - 2008/6/1
Y1 - 2008/6/1
N2 - Two-dimensional protein crystallization on lipid monolayers at a quiescent air/water interface is now a well-established process, but it only operates under a very restricted set of conditions and on a very slow time scale. We have recently been able to significantly extend the conditions under which the proteins will crystallize as well as speed up the process by subjecting the interface to a shearing flow. Here, we investigate the two-way coupling between a protein-laden film and the bulk flow that provides the interfacial shear. This flow in a stationary open cylinder is driven by the constant rotation of the floor. Using the Boussinesq-Scriven surface model for a Newtonian interface coupled to the Navier-Stokes equations for the bulk flow, we find that the surface shear viscosity of protein-laden films under most conditions is small or negligible. This is the case for films subjected to constant shearing flow, regardless of the duration of the flow. However, when the film is intermittently sheared, significant surface shear viscosity is evident. In these cases, the surface shear viscosity is not uniform across the film.
AB - Two-dimensional protein crystallization on lipid monolayers at a quiescent air/water interface is now a well-established process, but it only operates under a very restricted set of conditions and on a very slow time scale. We have recently been able to significantly extend the conditions under which the proteins will crystallize as well as speed up the process by subjecting the interface to a shearing flow. Here, we investigate the two-way coupling between a protein-laden film and the bulk flow that provides the interfacial shear. This flow in a stationary open cylinder is driven by the constant rotation of the floor. Using the Boussinesq-Scriven surface model for a Newtonian interface coupled to the Navier-Stokes equations for the bulk flow, we find that the surface shear viscosity of protein-laden films under most conditions is small or negligible. This is the case for films subjected to constant shearing flow, regardless of the duration of the flow. However, when the film is intermittently sheared, significant surface shear viscosity is evident. In these cases, the surface shear viscosity is not uniform across the film.
KW - Surface shear viscosity
KW - Two-dimensional protein crystallization
UR - http://www.scopus.com/inward/record.url?scp=43049125752&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=43049125752&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2008.02.059
DO - 10.1016/j.jcis.2008.02.059
M3 - Article
C2 - 18374937
AN - SCOPUS:43049125752
VL - 322
SP - 79
EP - 86
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
SN - 0021-9797
IS - 1
ER -