Copper(I)-bleomycin. A structurally unique oxidation-reduction active complex

N. J. Oppenheimer, C. Chang, L. O. Rodriguez, Sidney Hecht

Research output: Contribution to journalArticle

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Abstract

Cu(I) and Cu(II) form stable 1:1 complexes with bleomycin (BLM). The affinity of both metals for the drug is greater than that of Fe(II). Cu(I)-BLM A2 binds to calf thymus DNA with about the same affinity as Fe(II)-BLM, as judged by DNA-induced fluorescence quenching of the bithiazole moiety of BLM. Based on 1H NMR and potentiometric titration data, the Cu(I) complexes of BLM are shown to have geometries very different than those of other BLM-metal(II) complexes studied thus far. As Cu(I)-BLM is oxidation-reduction active, its geometry is of importance in defining the structural requirements for BLM activity.

Original languageEnglish (US)
Pages (from-to)1514-1517
Number of pages4
JournalJournal of Biological Chemistry
Volume256
Issue number4
StatePublished - 1981
Externally publishedYes

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Bleomycin
Oxidation-Reduction
Copper
Coordination Complexes
Geometry
Metal complexes
copper bleomycin
Titration
Fluorescence
Metals
Quenching
Nuclear magnetic resonance
DNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Biochemistry

Cite this

Copper(I)-bleomycin. A structurally unique oxidation-reduction active complex. / Oppenheimer, N. J.; Chang, C.; Rodriguez, L. O.; Hecht, Sidney.

In: Journal of Biological Chemistry, Vol. 256, No. 4, 1981, p. 1514-1517.

Research output: Contribution to journalArticle

Oppenheimer, NJ, Chang, C, Rodriguez, LO & Hecht, S 1981, 'Copper(I)-bleomycin. A structurally unique oxidation-reduction active complex', Journal of Biological Chemistry, vol. 256, no. 4, pp. 1514-1517.
Oppenheimer, N. J. ; Chang, C. ; Rodriguez, L. O. ; Hecht, Sidney. / Copper(I)-bleomycin. A structurally unique oxidation-reduction active complex. In: Journal of Biological Chemistry. 1981 ; Vol. 256, No. 4. pp. 1514-1517.
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