TY - JOUR
T1 - Coordinate inhibition of expression of several genes for protein subunits of human nuclear RNase P
AU - Kovrigina, Elizaveta
AU - Wesolowski, Donna
AU - Altman, Sidney
PY - 2003/2/18
Y1 - 2003/2/18
N2 - The deliberate inhibition of expression of one of the protein subunits (Rpp38) of human nuclear RNase P is achievable by using external guide sequence (EGS) technology. Both the protein product and the mRNA are greatly reduced 24 h after transient transfection with a gene coding for an appropriate EGS. Control experiments indicated that four other protein subunits of RNase P and their RNAs are also inhibited with no external manipulation. The remaining RNase P proteins, their mRNAs, and the RNA subunit of RNase P all are unchanged. Several short nucleotide sequences adjacent to the ORFs for the inhibited genes are similar and could be targets for transcriptional repression. The explanation of coordinate inhibition of the expression of the product of one particular gene by the transfection of an EGS (or RNA interference) requires some care in terms of interpreting phenotypic effects because, in our case, several gene products that are not targeted are also inhibited.
AB - The deliberate inhibition of expression of one of the protein subunits (Rpp38) of human nuclear RNase P is achievable by using external guide sequence (EGS) technology. Both the protein product and the mRNA are greatly reduced 24 h after transient transfection with a gene coding for an appropriate EGS. Control experiments indicated that four other protein subunits of RNase P and their RNAs are also inhibited with no external manipulation. The remaining RNase P proteins, their mRNAs, and the RNA subunit of RNase P all are unchanged. Several short nucleotide sequences adjacent to the ORFs for the inhibited genes are similar and could be targets for transcriptional repression. The explanation of coordinate inhibition of the expression of the product of one particular gene by the transfection of an EGS (or RNA interference) requires some care in terms of interpreting phenotypic effects because, in our case, several gene products that are not targeted are also inhibited.
KW - Downstream sequences
KW - External guide sequences
KW - Rpp38
KW - Upstream sequences
UR - http://www.scopus.com/inward/record.url?scp=0037452788&partnerID=8YFLogxK
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U2 - 10.1073/pnas.0337661100
DO - 10.1073/pnas.0337661100
M3 - Article
C2 - 12552092
AN - SCOPUS:0037452788
SN - 0027-8424
VL - 100
SP - 1598
EP - 1602
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 4
ER -