Coordinate inhibition of expression of several genes for protein subunits of human nuclear RNase P

Elizaveta Kovrigina, Donna Wesolowski, Sidney Altman

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The deliberate inhibition of expression of one of the protein subunits (Rpp38) of human nuclear RNase P is achievable by using external guide sequence (EGS) technology. Both the protein product and the mRNA are greatly reduced 24 h after transient transfection with a gene coding for an appropriate EGS. Control experiments indicated that four other protein subunits of RNase P and their RNAs are also inhibited with no external manipulation. The remaining RNase P proteins, their mRNAs, and the RNA subunit of RNase P all are unchanged. Several short nucleotide sequences adjacent to the ORFs for the inhibited genes are similar and could be targets for transcriptional repression. The explanation of coordinate inhibition of the expression of the product of one particular gene by the transfection of an EGS (or RNA interference) requires some care in terms of interpreting phenotypic effects because, in our case, several gene products that are not targeted are also inhibited.

Original languageEnglish (US)
Pages (from-to)1598-1602
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number4
DOIs
StatePublished - Feb 18 2003
Externally publishedYes

Keywords

  • Downstream sequences
  • External guide sequences
  • Rpp38
  • Upstream sequences

ASJC Scopus subject areas

  • General

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