Controlled human malaria infections by intradermal injection of cryopreserved Plasmodium falciparum sporozoites

Meta Roestenberg, Else M. Bijker, B. Kim Lee Sim, Peter F. Billingsley, Eric R. James, Guido J.H. Bastiaens, Anne C. Teirlinck, Anja Scholzen, Karina Teelen, Theo Arens, André J.A.M. Van Der Ven, Anusha Gunasekera, Sumana Chakravarty, Soundarapandian Velmurugan, Cornelus C. Hermsen, Robert W. Sauerwein, Stephen L. Hoffman

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

Controlled human malaria infection with sporozoites is a standardized and powerful tool for evaluation of malaria vaccine and drug efficacy but so far only applied by exposure to bites of Plasmodium falciparum (Pf)-infected mosquitoes. We assessed in an open label Phase 1 trial, infection after intradermal injection of respectively 2,500, 10,000, or 25,000 aseptic, purified, vialed, cryopreserved Pf sporozoites (PfSPZ) in three groups (N = 6/group) of healthy Dutch volunteers. Infection was safe and parasitemia developed in 15 of 18 volunteers (84%), 5 of 6 volunteers in each group. There were no differences between groups in time until parasitemia by microscopy or quantitative polymerase chain reaction, parasite kinetics, clinical symptoms, or laboratory values. This is the first successful infection by needle and syringe with PfSPZ manufactured in compliance with regulatory standards. After further optimization, the use of such PfSPZ may facilitate and accelerate clinical development of novel malaria drugs and vaccines.

Original languageEnglish (US)
Pages (from-to)5-13
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume88
Issue number1
DOIs
StatePublished - Jan 2013
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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