Controlled delivery of DNA origami on patterned surfaces

Aren E. Gerdon, Seung Soo Oh, Kuangwen Hsieh, Yonggang Ke, Hao Yan, H. Tom Soh

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

A strategy that combines top-down fabrication and bottom-up self-assembly to achieve deterministic position control of DNA origami structures over millimeter length scales on a wafer has been reported. A chemical strategy to attract and immobilize the origami onto gold surfaces using a carboxylic acid terminated self-assembled monolayer (SAM) has also been formulated. AFM micrographs revealed no DNA adsorption on SiO2 surfaces whereas origami structures were clearly visible on the gold surfaces. The substrate was stringently washed using 1× TAE buffer, to avoid any specific physiosorption of gold nanoparticles. AFM in both height and phase modes clearly confirmed the delivery of gold nanoparticles onto individual origami rectangles on gold patterns. The method exploits fundamental processes of magnesium chelation and base-pair hybridization, which is scalable, robust, and reproducible.

Original languageEnglish (US)
Pages (from-to)1942-1946
Number of pages5
JournalSmall
Volume5
Issue number17
DOIs
StatePublished - Sep 4 2009

Keywords

  • DNA
  • Nanoparticles
  • Origami
  • Self-assembly
  • Surface patterning

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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  • Cite this

    Gerdon, A. E., Oh, S. S., Hsieh, K., Ke, Y., Yan, H., & Soh, H. T. (2009). Controlled delivery of DNA origami on patterned surfaces. Small, 5(17), 1942-1946. https://doi.org/10.1002/smll.200900442