Control of the position of RNase P-mediated transfer RNA precursor processing

B. J. Carter, B. S. Vold, S. M. Hecht

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Two Bacillus subtilis tRNA(His) precursors (Green, C.J., and Vold, B.S. (1988) J. Biol. Chem. 263, 652-657) were processed by Escherichia coli RNase P in the presence of varying [Mg2+]. The wild type precursor was processed under all conditions to afford a single tRNA product containing 8 base pairs in the acceptor stem. In contrast, the position of processing of a mutant tRNA(His) precursor (containing a G27 ⇆ A27 alteration) was shown to be condition-dependent. Processing occurred at A27 under conditions consistent with formation of an A27-C100 base pair in the acceptor stem but at G28 under conditions that disfavored base pair formation. The ability to control the site of RNAse P-mediated tRNA precursor processing is unprecedented and permits analysis of the chemical factors that promote processing.

Original languageEnglish (US)
Pages (from-to)7100-71036
Number of pages63937
JournalJournal of Biological Chemistry
Volume265
Issue number13
StatePublished - Jun 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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