Two Bacillus subtilis tRNA(His) precursors (Green, C.J., and Vold, B.S. (1988) J. Biol. Chem. 263, 652-657) were processed by Escherichia coli RNase P in the presence of varying [Mg2+]. The wild type precursor was processed under all conditions to afford a single tRNA product containing 8 base pairs in the acceptor stem. In contrast, the position of processing of a mutant tRNA(His) precursor (containing a G27 ⇆ A27 alteration) was shown to be condition-dependent. Processing occurred at A27 under conditions consistent with formation of an A27-C100 base pair in the acceptor stem but at G28 under conditions that disfavored base pair formation. The ability to control the site of RNAse P-mediated tRNA precursor processing is unprecedented and permits analysis of the chemical factors that promote processing.
|Original language||English (US)|
|Number of pages||63937|
|Journal||Journal of Biological Chemistry|
|State||Published - Jun 1 1990|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology