Control of cell division in Saccharomyces cerevisiae by methionyl tRNA

M. W. Unger, Leland Hartwell

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84 Citations (Scopus)

Abstract

It is suggested that two events are necessary for an ansynchronous population of cells to undergo arrest in the G1 phase of the cell cycle upon nutrient starvation. First, passage through G1 must be prevented by a deficiency of some metabolic intermediate. Since this intermediate may act indirectly to arrest division, we designate it the 'signal'. Three conditions were found under which S. cerevisiae cells arrest division in G1: sulfate starvation of a prototroph, methionine starvation of an auxotroph, or a shift of a conditional methionyl tRNA synthetase mutant [L methionine:tRNA(Met) ligase (AMP forming), EC 6.1.1.10] to a restrictive condition. These results indicate that the signal for sulfate starvation in S. cerevisiae is generated near the end of the sulfate assimilation pathway (at or beyond the formation of methionyl tRNA). It is proposed that the signal for all nutrients is generated at the level of protein biosynthesis. A second event necessary for G1 arrest is the provision of sufficient protein synthetic capacity for cells to finish the cycles that are in progress when the signal is generated. This necessity is demonstrated by the failure of the methionyl tRNA synthetase mutant to undergo G1 arrest when protein synthesis is abruptly terminated by a shift to 36° into methionine deficient medium.

Original languageEnglish (US)
Pages (from-to)1664-1668
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number5
StatePublished - 1976
Externally publishedYes

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Methionine-tRNA Ligase
Starvation
Transfer RNA
Cell Division
Saccharomyces cerevisiae
Sulfates
Methionine
Artificial Cells
Food
G1 Phase
Protein Biosynthesis
Cell Cycle
Proteins
Population

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "It is suggested that two events are necessary for an ansynchronous population of cells to undergo arrest in the G1 phase of the cell cycle upon nutrient starvation. First, passage through G1 must be prevented by a deficiency of some metabolic intermediate. Since this intermediate may act indirectly to arrest division, we designate it the 'signal'. Three conditions were found under which S. cerevisiae cells arrest division in G1: sulfate starvation of a prototroph, methionine starvation of an auxotroph, or a shift of a conditional methionyl tRNA synthetase mutant [L methionine:tRNA(Met) ligase (AMP forming), EC 6.1.1.10] to a restrictive condition. These results indicate that the signal for sulfate starvation in S. cerevisiae is generated near the end of the sulfate assimilation pathway (at or beyond the formation of methionyl tRNA). It is proposed that the signal for all nutrients is generated at the level of protein biosynthesis. A second event necessary for G1 arrest is the provision of sufficient protein synthetic capacity for cells to finish the cycles that are in progress when the signal is generated. This necessity is demonstrated by the failure of the methionyl tRNA synthetase mutant to undergo G1 arrest when protein synthesis is abruptly terminated by a shift to 36° into methionine deficient medium.",
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N2 - It is suggested that two events are necessary for an ansynchronous population of cells to undergo arrest in the G1 phase of the cell cycle upon nutrient starvation. First, passage through G1 must be prevented by a deficiency of some metabolic intermediate. Since this intermediate may act indirectly to arrest division, we designate it the 'signal'. Three conditions were found under which S. cerevisiae cells arrest division in G1: sulfate starvation of a prototroph, methionine starvation of an auxotroph, or a shift of a conditional methionyl tRNA synthetase mutant [L methionine:tRNA(Met) ligase (AMP forming), EC 6.1.1.10] to a restrictive condition. These results indicate that the signal for sulfate starvation in S. cerevisiae is generated near the end of the sulfate assimilation pathway (at or beyond the formation of methionyl tRNA). It is proposed that the signal for all nutrients is generated at the level of protein biosynthesis. A second event necessary for G1 arrest is the provision of sufficient protein synthetic capacity for cells to finish the cycles that are in progress when the signal is generated. This necessity is demonstrated by the failure of the methionyl tRNA synthetase mutant to undergo G1 arrest when protein synthesis is abruptly terminated by a shift to 36° into methionine deficient medium.

AB - It is suggested that two events are necessary for an ansynchronous population of cells to undergo arrest in the G1 phase of the cell cycle upon nutrient starvation. First, passage through G1 must be prevented by a deficiency of some metabolic intermediate. Since this intermediate may act indirectly to arrest division, we designate it the 'signal'. Three conditions were found under which S. cerevisiae cells arrest division in G1: sulfate starvation of a prototroph, methionine starvation of an auxotroph, or a shift of a conditional methionyl tRNA synthetase mutant [L methionine:tRNA(Met) ligase (AMP forming), EC 6.1.1.10] to a restrictive condition. These results indicate that the signal for sulfate starvation in S. cerevisiae is generated near the end of the sulfate assimilation pathway (at or beyond the formation of methionyl tRNA). It is proposed that the signal for all nutrients is generated at the level of protein biosynthesis. A second event necessary for G1 arrest is the provision of sufficient protein synthetic capacity for cells to finish the cycles that are in progress when the signal is generated. This necessity is demonstrated by the failure of the methionyl tRNA synthetase mutant to undergo G1 arrest when protein synthesis is abruptly terminated by a shift to 36° into methionine deficient medium.

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