Contrast and radiation damage in point-projection electron imaging of purple membrane at 100 V

John Spence, W. Qian, X. Zhang

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Experimental transmission point-projection images of edge regions of uncoated, unstained purple membrane (PM) protein have been obtained at a beam energy of 100 V. We find that very high electron doses may be tolerated at this electron energy without structural change at the 2 nm level, that the membrane (thickness about 5.0 nm) is transparent above 1 kV and opaque at 100 V, and that is shows no charging effects below about 200 V. Image interpretation and damage mechanisms are reviewed.

Original languageEnglish (US)
Pages (from-to)19-23
Number of pages5
JournalUltramicroscopy
Volume55
Issue number1
DOIs
StatePublished - Jul 1994

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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