Contrast and radiation damage in point-projection electron imaging of purple membrane at 100 V

John Spence, W. Qian, X. Zhang

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Experimental transmission point-projection images of edge regions of uncoated, unstained purple membrane (PM) protein have been obtained at a beam energy of 100 V. We find that very high electron doses may be tolerated at this electron energy without structural change at the 2 nm level, that the membrane (thickness about 5.0 nm) is transparent above 1 kV and opaque at 100 V, and that is shows no charging effects below about 200 V. Image interpretation and damage mechanisms are reviewed.

Original languageEnglish (US)
Pages (from-to)19-23
Number of pages5
JournalUltramicroscopy
Volume55
Issue number1
DOIs
StatePublished - 1994

Fingerprint

Radiation damage
radiation damage
projection
membranes
damage
Membranes
Imaging techniques
Electrons
charging
Membrane Proteins
electrons
electron energy
proteins
Proteins
dosage
energy

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation

Cite this

Contrast and radiation damage in point-projection electron imaging of purple membrane at 100 V. / Spence, John; Qian, W.; Zhang, X.

In: Ultramicroscopy, Vol. 55, No. 1, 1994, p. 19-23.

Research output: Contribution to journalArticle

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