Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

J. O. Mascorro-Gallardo, A. A. Covarrubias, R. Gaxiola

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

Original languageEnglish (US)
Pages (from-to)169-170
Number of pages2
JournalGene
Volume172
Issue number1
DOIs
StatePublished - Jun 12 1996
Externally publishedYes

Keywords

  • Centromeric plasmid
  • Recombinant DNA
  • Yeast shuttle-expression vector

ASJC Scopus subject areas

  • Genetics

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