Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

J. O. Mascorro-Gallardo; A. A. Covarrubias; R. Gaxiola

doi:10.1016/0378-1119(96)00059-5

Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

J. O. Mascorro-Gallardo, A. A. Covarrubias, R. Gaxiola

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

Original language	English (US)
Pages (from-to)	169-170
Number of pages	2
Journal	Gene
Volume	172
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(96)00059-5
State	Published - Jun 12 1996
Externally published	Yes

Keywords

Centromeric plasmid
Recombinant DNA
Yeast shuttle-expression vector

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(96)00059-5

Cite this

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title = "Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae",

abstract = "We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.",

keywords = "Centromeric plasmid, Recombinant DNA, Yeast shuttle-expression vector",

author = "Mascorro-Gallardo, {J. O.} and Covarrubias, {A. A.} and R. Gaxiola",

note = "Funding Information: We thank Roberto Coria and Mario Zurita for kind advice. We also thank Paul Gaytan from the Unit of Chemical Synthesis of Macromolecules (IBT-UNAM) for synthesizing the oligos. This project was supported in part by the Consejo National de Ciencia y Tecnologia, MCxico (3460-N9310), and by the Organization of European Communities to R.G. (CIl*CT94-0082).",

year = "1996",

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doi = "10.1016/0378-1119(96)00059-5",

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T1 - Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

AU - Mascorro-Gallardo, J. O.

AU - Covarrubias, A. A.

AU - Gaxiola, R.

N1 - Funding Information: We thank Roberto Coria and Mario Zurita for kind advice. We also thank Paul Gaytan from the Unit of Chemical Synthesis of Macromolecules (IBT-UNAM) for synthesizing the oligos. This project was supported in part by the Consejo National de Ciencia y Tecnologia, MCxico (3460-N9310), and by the Organization of European Communities to R.G. (CIl*CT94-0082).

PY - 1996/6/12

Y1 - 1996/6/12

N2 - We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

AB - We report the construction and characterization of a yeast shuttle-expression vector that can be used to express genes in yeast in a modulated form. This vector is centromeric, with a polylinker that optionally can provide an ATG start of translation. Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.

KW - Centromeric plasmid

KW - Recombinant DNA

KW - Yeast shuttle-expression vector

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Construction of a CUP1 promoter-based vector to modulate gene expression in Saccharomyces cerevisiae

Abstract

Keywords

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