Construction and Serological Characterization of a Recombinant Human Single Chain T-Cell Receptor

Douglas Lake, R. M. Bernstein, E. M. Hersh, H. Kaymaz, S. F. Schluter, J. J. Marchalonis

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A single chain T cell receptor (scTcr) was constructed from the complete Vα and Vβ regions of Jurkat T-cell receptor α/β chain genes using molecular cloning techniques. The recombinant scTcr reacted with a panel of rabbit antisera generated against synthetic 16-mer peptides duplicating the amino acid sequence of Jurkat Vα and β chains but not with antisera directed against peptides from the constant domain. Autoantibodies present in sera from systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients bound the scTcr in ELISA assays. The recombinant scTcr described here should prove to be a useful reagent with which to study T-cell receptor activity in serological and functional assays.

Original languageEnglish (US)
Pages (from-to)1502-1509
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume201
Issue number3
DOIs
StatePublished - Jun 30 1994
Externally publishedYes

Fingerprint

T-Cell Antigen Receptor
Immune Sera
Assays
T-Cell Receptor Genes
Jurkat Cells
Molecular Cloning
Peptides
Cloning
Systemic Lupus Erythematosus
Autoantibodies
Amino Acid Sequence
Rheumatoid Arthritis
Enzyme-Linked Immunosorbent Assay
Rabbits
Genes
Amino Acids
Serum

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Construction and Serological Characterization of a Recombinant Human Single Chain T-Cell Receptor. / Lake, Douglas; Bernstein, R. M.; Hersh, E. M.; Kaymaz, H.; Schluter, S. F.; Marchalonis, J. J.

In: Biochemical and Biophysical Research Communications, Vol. 201, No. 3, 30.06.1994, p. 1502-1509.

Research output: Contribution to journalArticle

Lake, Douglas ; Bernstein, R. M. ; Hersh, E. M. ; Kaymaz, H. ; Schluter, S. F. ; Marchalonis, J. J. / Construction and Serological Characterization of a Recombinant Human Single Chain T-Cell Receptor. In: Biochemical and Biophysical Research Communications. 1994 ; Vol. 201, No. 3. pp. 1502-1509.
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