Construction and in vivo testing of prokaryotic riboregulators

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNAs that are transcribed and self-assemble within living cells are valuable tools for regulating and organizing cellular activities. Riboregulators, in particular, have been widely used for modulating translation and transcription in response to cognate transactivating or trigger RNAs, enabling cells to evaluate logic operations and to respond to environmental cues. Herein we detail a set of methods for the rapid construction and testing of prokaryotic riboregulators in Escherichia coli. These methods enable construction of dozens of riboregulator plasmids at the same time without the use of restriction enzymes. Furthermore, they facilitate rapid screening of devices and can be applied to a variety of other self-assembling in vivo RNA systems.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages285-302
Number of pages18
Volume1632
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1632
ISSN (Print)1064-3745

Fingerprint

RNA
Cues
Plasmids
Escherichia coli
Equipment and Supplies
Enzymes

Keywords

  • Cloning
  • Diagnostic
  • Plasmid
  • Regulation
  • Riboregulator
  • Riboswitch
  • Self-assembly
  • Sensor
  • Toehold switch
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Green, A. (2017). Construction and in vivo testing of prokaryotic riboregulators. In Methods in Molecular Biology (Vol. 1632, pp. 285-302). (Methods in Molecular Biology; Vol. 1632). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7138-1_19

Construction and in vivo testing of prokaryotic riboregulators. / Green, Alexander.

Methods in Molecular Biology. Vol. 1632 Humana Press Inc., 2017. p. 285-302 (Methods in Molecular Biology; Vol. 1632).

Research output: Chapter in Book/Report/Conference proceedingChapter

Green, A 2017, Construction and in vivo testing of prokaryotic riboregulators. in Methods in Molecular Biology. vol. 1632, Methods in Molecular Biology, vol. 1632, Humana Press Inc., pp. 285-302. https://doi.org/10.1007/978-1-4939-7138-1_19
Green A. Construction and in vivo testing of prokaryotic riboregulators. In Methods in Molecular Biology. Vol. 1632. Humana Press Inc. 2017. p. 285-302. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7138-1_19
Green, Alexander. / Construction and in vivo testing of prokaryotic riboregulators. Methods in Molecular Biology. Vol. 1632 Humana Press Inc., 2017. pp. 285-302 (Methods in Molecular Biology).
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