Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: Cell surface polysaccharide as an anchorage for gingipanis

Mikio Shoji, Dinath B. Ratnayake, Yixin Shi, Tomoko Kadowaki, Kenji Yamamoto, Fuminobu Yoshimura, Akifumi Akamine, Michael A. Curtis, Koji Nakayama

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Abstract

A nonpigmented mutant of Porphyromonas gingivalis was constructed by using transposon mutagenesis. The mutant possessed the transposon DNA at the novel gene porR. Gene targeted mutagenesis revealed that porR was responsible for pigmentation. The porR gene shared similarities with genes of the degT family, the products of which are now considered to be transaminases involved in biosynthesis of sugar portions of cell-surface polysaccharides and aminoglycosides. The porR mutant showed a pleiotropic phenotype: delayed maturation of fimbrillin, preferential presence of Rgp and Kgp proteinases in culture supernatants, and no haemagglutination. The porR mutant had altered phenol extractable polysaccharide compared to the porR+ sibling strain. A mAb, 1B5, that reacts with sugar portions of P. gingivalis cell surface polysaccharide and membrane-type Rgp proteinase showed no reaction with the cell lysates of the porR mutant. These results indicate that porR is involved in biosynthesis of cell surface polysaccharide that may function as an anchorage for Rgp, Kgp, haemagglutinins and the haemoglobin receptor protein.

Original languageEnglish (US)
Pages (from-to)1183-1191
Number of pages9
JournalMicrobiology
Volume148
Issue number4
StatePublished - May 6 2002

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Keywords

  • Cell surface polysaccharide
  • Colonial pigmentation
  • Cysteine proteinases
  • Fimbrillin maturation
  • Haemogglutination

ASJC Scopus subject areas

  • Microbiology

Cite this

Shoji, M., Ratnayake, D. B., Shi, Y., Kadowaki, T., Yamamoto, K., Yoshimura, F., Akamine, A., Curtis, M. A., & Nakayama, K. (2002). Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: Cell surface polysaccharide as an anchorage for gingipanis. Microbiology, 148(4), 1183-1191.