Construction and characterization of a BAC-library for a key pollinator, the bumblebee Bombus terrestris L.

L. Wilfert, M. Muñoz Torres, C. Reber-Funk, R. Schmid-Hempel, J. Tomkins, Juergen Gadau, P. Schmid-Hempel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The primitively social bumblebee Bombus terrestris is an ecological model species as well as an important agricultural pollinator. As part of the ongoing development of genomic resources for this model organism, we have constructed a publicly available bacterial artificial chromosome (BAC) library from males of a field-derived colony. We have shown that this library has a high coverage, which allows any particular sequence to be retrieved from at least one clone with a probability of 99.7%. We have further demonstrated the library's usefulness by successfully screening it with probes derived both from previously described B. terrestris genes and candidate genes from another bumblebee species and the honeybee. This library will facilitate genomic studies in B. terrestris and will allow for novel comparative studies in the social Hymenoptera.

Original languageEnglish (US)
Pages (from-to)44-48
Number of pages5
JournalInsectes Sociaux
Volume56
Issue number1
DOIs
StatePublished - Feb 1 2009

Keywords

  • Breeding
  • Gene discovery
  • Genomics
  • Pollination
  • Sex determination

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

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    Wilfert, L., Torres, M. M., Reber-Funk, C., Schmid-Hempel, R., Tomkins, J., Gadau, J., & Schmid-Hempel, P. (2009). Construction and characterization of a BAC-library for a key pollinator, the bumblebee Bombus terrestris L. Insectes Sociaux, 56(1), 44-48. https://doi.org/10.1007/s00040-008-1034-1