Considerations for Low-cost Reader Design and Label Selection for Lateral Flow Assays

Uwadiae Obahiagbon, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We present a low-cost fluorescence and colorimetry label detection platform for point-of-care (POC) applications. Our platform is designed to deliver clinical level diagnostic sensitivity. It improves the performance of the traditional lateral flow assay (LFA) by decreasing the detection limit by 3 orders of magnitude and enabling quantitative detection of analytes. In this work, we characterize the fluorescence detection platform and detail the effects of label selection on the overall system performance. The effects of optoelectronic configuration, brightness, and spectral matching are presented. Finally, we demonstrate the utility of our platform for colorimetric detection and compare its performance to a standard central laboratory microplate reader. The limit-of-detection (LOD) for our point-of-care platform was determined to be the same as the microplate reader, detecting 2 × 104 0.5 μm microspheres without the use of focusing or transfer optics.

Original languageEnglish (US)
Title of host publication2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781538636039
DOIs
StatePublished - Dec 20 2018
Event2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018 - Cleveland, United States
Duration: Oct 17 2018Oct 19 2018

Other

Other2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018
CountryUnited States
CityCleveland
Period10/17/1810/19/18

Keywords

  • Colorimetry
  • Fluorescence
  • Lateral flow assay
  • Limit-of-detection
  • Point-of-care
  • Sensitivity

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Health Informatics
  • Instrumentation
  • Signal Processing
  • Biomedical Engineering

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