Considerations for Low-cost Reader Design and Label Selection for Lateral Flow Assays

Uwadiae Obahiagbon; Jennifer Blain Christen

doi:10.1109/BIOCAS.2018.8584842

Considerations for Low-cost Reader Design and Label Selection for Lateral Flow Assays

Uwadiae Obahiagbon, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

We present a low-cost fluorescence and colorimetry label detection platform for point-of-care (POC) applications. Our platform is designed to deliver clinical level diagnostic sensitivity. It improves the performance of the traditional lateral flow assay (LFA) by decreasing the detection limit by 3 orders of magnitude and enabling quantitative detection of analytes. In this work, we characterize the fluorescence detection platform and detail the effects of label selection on the overall system performance. The effects of optoelectronic configuration, brightness, and spectral matching are presented. Finally, we demonstrate the utility of our platform for colorimetric detection and compare its performance to a standard central laboratory microplate reader. The limit-of-detection (LOD) for our point-of-care platform was determined to be the same as the microplate reader, detecting 2 × 10⁴ 0.5 μm microspheres without the use of focusing or transfer optics.

Original language	English (US)
Title of host publication	2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018 - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781538636039
DOIs	https://doi.org/10.1109/BIOCAS.2018.8584842
State	Published - Dec 20 2018
Event	2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018 - Cleveland, United States Duration: Oct 17 2018 → Oct 19 2018

Other

Other	2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018
Country/Territory	United States
City	Cleveland
Period	10/17/18 → 10/19/18

Keywords

Colorimetry
Fluorescence
Lateral flow assay
Limit-of-detection
Point-of-care
Sensitivity

ASJC Scopus subject areas

Electrical and Electronic Engineering
Health Informatics
Instrumentation
Signal Processing
Biomedical Engineering

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10.1109/BIOCAS.2018.8584842

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Considerations for Low-cost Reader Design and Label Selection for Lateral Flow Assays. / Obahiagbon, Uwadiae; Blain Christen, Jennifer.
2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018 - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2018. 8584842.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Obahiagbon, U & Blain Christen, J 2018, Considerations for Low-cost Reader Design and Label Selection for Lateral Flow Assays. in 2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018 - Proceedings., 8584842, Institute of Electrical and Electronics Engineers Inc., 2018 IEEE Biomedical Circuits and Systems Conference, BioCAS 2018, Cleveland, United States, 10/17/18. https://doi.org/10.1109/BIOCAS.2018.8584842

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Considerations for Low-cost Reader Design and Label Selection for Lateral Flow Assays

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Keywords

ASJC Scopus subject areas

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