Conservation of distantly related membrane proteins: Photosynthetic reaction centers share a common structural core

Sumedha Sadekar, Jason Raymond, Robert E. Blankenship

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Photosynthesis was established on Earth more than 3 billion years ago. All available evidences suggest that the earliest photosynthetic organisms were anoxygenic and that oxygen-evolving photosynthesis is a more recent development. The reaction center complexes that form the heart of the energy storage process are integral membrane pigment proteins that span the membrane in vectorial fashion to carry out electron transfer. The origin and extent of distribution of these proteins has been perplexing from a phylogenetic point of view mostly because of extreme sequence divergence. A series of integral membrane proteins of known structure and varying degrees of sequence identity have been compared using combinatorial extension-Monte Carlo methods. The proteins include photosynthetic reaction centers from proteobacteria and cyanobacterial photosystems I and II, as well as cytochrome oxidase, bacteriorhodopsin, and cytochrome b. The reaction center complexes show a remarkable conservation of the core structure of 5 transmembrane helices, strongly implying common ancestry, even though the residual sequence identity is less than 10%, whereas the other proteins have structures that are unrelated. A relationship of sequence with structure was derived from the reaction center structures; with characteristic decay length of 1.6 Å. Phylogenetic trees derived from the structural alignments give insights into the earliest photosynthetic reaction center, strongly suggesting that it was a homodimeric complex that did not evolve oxygen.

Original languageEnglish (US)
Pages (from-to)2001-2007
Number of pages7
JournalMolecular Biology and Evolution
Volume23
Issue number11
DOIs
StatePublished - Nov 2006
Externally publishedYes

Fingerprint

photosynthetic reaction centers
Photosynthetic Reaction Center Complex Proteins
membrane proteins
Conservation
Membrane Proteins
Photosynthesis
protein structure
membrane
protein
photosynthesis
Oxygen
Bacteriorhodopsins
oxygen
Photosystem I Protein Complex
Monte Carlo Method
Proteobacteria
Proteins
Monte Carlo method
Cytochromes b
Photosystem II Protein Complex

Keywords

  • Evolution
  • Photosynthesis
  • Protein structure
  • Reaction center

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Genetics(clinical)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Ecology, Evolution, Behavior and Systematics
  • Agricultural and Biological Sciences (miscellaneous)
  • Molecular Biology

Cite this

Conservation of distantly related membrane proteins : Photosynthetic reaction centers share a common structural core. / Sadekar, Sumedha; Raymond, Jason; Blankenship, Robert E.

In: Molecular Biology and Evolution, Vol. 23, No. 11, 11.2006, p. 2001-2007.

Research output: Contribution to journalArticle

Sadekar, Sumedha ; Raymond, Jason ; Blankenship, Robert E. / Conservation of distantly related membrane proteins : Photosynthetic reaction centers share a common structural core. In: Molecular Biology and Evolution. 2006 ; Vol. 23, No. 11. pp. 2001-2007.
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