Conjecture regarding posttranslational modifications to the Arabidopsis type I Proton-pumping pyrophosphatase (AVP1)

Gaston A. Pizzio; Kendal D. Hirschi; Roberto Gaxiola

doi:10.3389/fpls.2017.01572

Conjecture regarding posttranslational modifications to the Arabidopsis type I Proton-pumping pyrophosphatase (AVP1)

Gaston A. Pizzio, Kendal D. Hirschi, Roberto Gaxiola

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Agbiotechnology uses genetic engineering to improve the output and value of crops. Altering the expression of the plant Type I Proton-pumping Pyrophosphatase (HC- PPase) has already proven to be a useful tool to enhance crop productivity. Despite the effective use of this gene in translational research, information regarding the intracellular localization and functional plasticity of the pump remain largely enigmatic. Using computer modeling several putative phosphorylation, ubiquitination and sumoylation target sites were identified that may regulate Arabidopsis HC-PPase (AVP1- Arabidopsis Vacuolar Proton-pump 1) subcellular trafficking and activity. These putative regulatory sites will direct future research that specifically addresses the partitioning and transport characteristics of this pump. We posit that fine-tuning HC-PPases activity and cellular distribution will facilitate rationale strategies for further genetic improvements in crop productivity.

Original language	English (US)
Article number	1572
Journal	Frontiers in Plant Science
Volume	8
DOIs	https://doi.org/10.3389/fpls.2017.01572
State	Published - Sep 12 2017

Keywords

AVP1
H-PPase
Phosphorylation
Structural modelling
Sumoylation
Ubiquitination

ASJC Scopus subject areas

Plant Science

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10.3389/fpls.2017.01572

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title = "Conjecture regarding posttranslational modifications to the Arabidopsis type I Proton-pumping pyrophosphatase (AVP1)",

abstract = "Agbiotechnology uses genetic engineering to improve the output and value of crops. Altering the expression of the plant Type I Proton-pumping Pyrophosphatase (HC- PPase) has already proven to be a useful tool to enhance crop productivity. Despite the effective use of this gene in translational research, information regarding the intracellular localization and functional plasticity of the pump remain largely enigmatic. Using computer modeling several putative phosphorylation, ubiquitination and sumoylation target sites were identified that may regulate Arabidopsis HC-PPase (AVP1- Arabidopsis Vacuolar Proton-pump 1) subcellular trafficking and activity. These putative regulatory sites will direct future research that specifically addresses the partitioning and transport characteristics of this pump. We posit that fine-tuning HC-PPases activity and cellular distribution will facilitate rationale strategies for further genetic improvements in crop productivity.",

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KW - Structural modelling

KW - Sumoylation

KW - Ubiquitination

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Conjecture regarding posttranslational modifications to the Arabidopsis type I Proton-pumping pyrophosphatase (AVP1)

Abstract

Keywords

ASJC Scopus subject areas

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