Conformational transition in DNA on a cold surface

X. Z. Feng, R. Bash, P. Balagurumoorthy, D. Lohr, R. E. Harrington, Stuart Lindsay

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The contour length of DNA fragments, deposited and imaged on mica under buffer, was measured as a function of deposition temperature. Extended DNA molecules (on Ni- and silane-treated surfaces) contract rapidly with falling temperature, approaching the contour length of A-DNA at 2°C. The contraction is not unique to a specific sequence and does not occur in solution at 2°C or on a surface at 25°C, indicating that it arises from a combination of low temperature and surface contact. It is probably a consequence of reduced water activity at a cold surface.

Original languageEnglish (US)
Pages (from-to)593-596
Number of pages4
JournalNucleic Acids Research
Volume28
Issue number2
StatePublished - Jan 15 2000

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Temperature
DNA
A-Form DNA
Silanes
Buffers
Water
mica

ASJC Scopus subject areas

  • Genetics

Cite this

Feng, X. Z., Bash, R., Balagurumoorthy, P., Lohr, D., Harrington, R. E., & Lindsay, S. (2000). Conformational transition in DNA on a cold surface. Nucleic Acids Research, 28(2), 593-596.

Conformational transition in DNA on a cold surface. / Feng, X. Z.; Bash, R.; Balagurumoorthy, P.; Lohr, D.; Harrington, R. E.; Lindsay, Stuart.

In: Nucleic Acids Research, Vol. 28, No. 2, 15.01.2000, p. 593-596.

Research output: Contribution to journalArticle

Feng, XZ, Bash, R, Balagurumoorthy, P, Lohr, D, Harrington, RE & Lindsay, S 2000, 'Conformational transition in DNA on a cold surface', Nucleic Acids Research, vol. 28, no. 2, pp. 593-596.
Feng XZ, Bash R, Balagurumoorthy P, Lohr D, Harrington RE, Lindsay S. Conformational transition in DNA on a cold surface. Nucleic Acids Research. 2000 Jan 15;28(2):593-596.
Feng, X. Z. ; Bash, R. ; Balagurumoorthy, P. ; Lohr, D. ; Harrington, R. E. ; Lindsay, Stuart. / Conformational transition in DNA on a cold surface. In: Nucleic Acids Research. 2000 ; Vol. 28, No. 2. pp. 593-596.
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