Conformational landscape of a virus by single-particle X-ray scattering

Ahmad Hosseinizadeh, Ghoncheh Mashayekhi, Jeremy Copperman, Peter Schwander, Ali Dashti, Reyhaneh Sepehr, Russell Fung, Marius Schmidt, Chun Hong Yoon, Brenda Hogue, Garth J. Williams, Andrew Aquila, Abbas Ourmazd

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Using a manifold-based analysis of experimental diffraction snapshots from an X-ray free electron laser, we determine the three-dimensional structure and conformational landscape of the PR772 virus to a detector-limited resolution of 9 nm. Our results indicate that a single conformational coordinate controls reorganization of the genome, growth of a tubular structure from a portal vertex and release of the genome. These results demonstrate that single-particle X-ray scattering has the potential to shed light on key biological processes.

Original languageEnglish (US)
Pages (from-to)877-881
Number of pages5
JournalNature Methods
Volume14
Issue number9
DOIs
StatePublished - Aug 31 2017

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

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    Hosseinizadeh, A., Mashayekhi, G., Copperman, J., Schwander, P., Dashti, A., Sepehr, R., Fung, R., Schmidt, M., Yoon, C. H., Hogue, B., Williams, G. J., Aquila, A., & Ourmazd, A. (2017). Conformational landscape of a virus by single-particle X-ray scattering. Nature Methods, 14(9), 877-881. https://doi.org/10.1038/nmeth.4395