Conformation of Amine-Modified DNA: 2-Aminofluorene- and 2-(Acetylamino)fluorene-Modified Deoxydinucleoside Monophosphates with All Possible Nearest Neighbors. A Comparison of Search and Optimization Methods

Robert Shapiro, Dany Sidawi, Yong Su Miao, Brian E. Hingerty, Kevin Schmidt, Jules Moskowitz, Suse Broyde

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Abstract

Although a significant part of the replication fork exists as single-stranded DNA, little is known about the effect of carcinogens and mutagens on single-strand conformation. Large scale conformational searches with potential energy minimization, using the torsion angle space molecular mechanics program DUPLEX, were employed to explore the conformation of all 16 deoxydinucleoside monophosphates bearing 2-aminofluorene (AF) or 2-(acetylamino)fluorene (AAF) modification on guanine. We have thus examined the effect of 3′ versus 5′ modification, the presence or absence of the acetyl group, and the effect of four different neighbors in each case. The principal effect of the acetyl group appeared to be the destabilization of anti (and, to a lesser degree, borderline anti) conformations for modified guanine. This mattered little in the 5′-substituted dimers, where one conformational type predominated in the low-energy structures for the adducts of both AAF and AF: It was right-handed, with syn-guanine, imperfect base–base stacking, and fluorene to 3′-sugar contacts. Greater divergence was seen in the 3′-substituted series. The AAF-substituted 3′-adducts primarily displayed good base-fluorene stacking, with syn-guanine in contact with the 5′-sugar. The AF-substituted 3′-adducts displayed a variety of structures which included base-base and carcinogen-base stacked forms. Two novel forms were encountered [global minima for d(ApG–AF) and d(GpG–AF)], whose unusual structures suggest mutagenic capability. In order to address the multiple minimum problem, we conducted our searches of conformation space using two alternative optimization methods that also employ differing search strategies. We used the Powell algorithm, BOTM, with starting conformations that are selected combinations of rotamers, and the method of simulated annealing (SA), with random or arbitrary starting conformations. While both approaches were effective in defining the most important structures, SA was more successful than BOTM in locating the structures of lowest energy.

Original languageEnglish (US)
Pages (from-to)239-253
Number of pages15
JournalChemical Research in Toxicology
Volume7
Issue number2
DOIs
StatePublished - Mar 1 1994

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ASJC Scopus subject areas

  • Toxicology

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