Conductometric monitoring of protein-protein interactions

Rosanna Spera, Fernanda Festa, Nicola L. Bragazzi, Eugenia Pechkova, Joshua LaBaer, Claudio Nicolini

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Conductometric monitoring of protein-protein and protein-sterol interactions is here proved feasible by coupling quartz crystal microbalance with dissipation monitoring (QCM-D) to nucleic acid programmable protein arrays (NAPPA). The conductance curves measured in NAPPA microarrays printed on quartz surface allowed the identification of binding events between the immobilized proteins and the query. NAPPA allows the immobilization on the quartz surface of a wide range of proteins and can be easily adapted to generate innumerous types of biosensors. Indeed multiple proteins on the same quartz crystal have been tested and envisaged proving the possibility of analyzing the same array for several distinct interactions. Two examples of NAPPA-based conductometer applications with clinical relevance are presented herein, the interaction between the transcription factors Jun and ATF2 and the interaction between Cytochrome P540scc and cholesterol.

Original languageEnglish (US)
Pages (from-to)5535-5547
Number of pages13
JournalJournal of Proteome Research
Volume12
Issue number12
DOIs
StatePublished - Dec 6 2013

Fingerprint

Protein Array Analysis
Nucleic Acids
Quartz
Monitoring
Proteins
Activating Transcription Factor 2
Quartz Crystal Microbalance Techniques
Immobilized Proteins
Biosensing Techniques
Sterols
Cytochromes
Immobilization
Cholesterol
Quartz crystal microbalances
Microarrays
Biosensors
Transcription Factors
Crystals

Keywords

  • cell free expression system
  • conductometer
  • nucleic acid programmable protein array
  • quartz crystal microbalance with dissipation monitoring

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Medicine(all)

Cite this

Spera, R., Festa, F., Bragazzi, N. L., Pechkova, E., LaBaer, J., & Nicolini, C. (2013). Conductometric monitoring of protein-protein interactions. Journal of Proteome Research, 12(12), 5535-5547. https://doi.org/10.1021/pr400445v

Conductometric monitoring of protein-protein interactions. / Spera, Rosanna; Festa, Fernanda; Bragazzi, Nicola L.; Pechkova, Eugenia; LaBaer, Joshua; Nicolini, Claudio.

In: Journal of Proteome Research, Vol. 12, No. 12, 06.12.2013, p. 5535-5547.

Research output: Contribution to journalArticle

Spera, R, Festa, F, Bragazzi, NL, Pechkova, E, LaBaer, J & Nicolini, C 2013, 'Conductometric monitoring of protein-protein interactions', Journal of Proteome Research, vol. 12, no. 12, pp. 5535-5547. https://doi.org/10.1021/pr400445v
Spera R, Festa F, Bragazzi NL, Pechkova E, LaBaer J, Nicolini C. Conductometric monitoring of protein-protein interactions. Journal of Proteome Research. 2013 Dec 6;12(12):5535-5547. https://doi.org/10.1021/pr400445v
Spera, Rosanna ; Festa, Fernanda ; Bragazzi, Nicola L. ; Pechkova, Eugenia ; LaBaer, Joshua ; Nicolini, Claudio. / Conductometric monitoring of protein-protein interactions. In: Journal of Proteome Research. 2013 ; Vol. 12, No. 12. pp. 5535-5547.
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