Computational model of optogenetic stimulation in a peripheral nerve

Nicholas Fritz, Daniel Gulick, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Stimulation has been a key technique for studying underlying mechanisms of the nervous system. Electrical stimulation has been the predominant method for eliciting desired muscle responses for decades, yet methodologies remain invasive and low in selectivity of tissue stimulated. Current injection affects all local tissue types and can lead to damaging immune responses that threaten both nerves and equipment alike. Optogenetics provides a solution for such stimulation difficulties by increasing specificity and decreasing risk to tissue. Via genetic modifications, opsins (light-sensitive proteins) are added to neurons, and can be activated by light to cause neuron excitation. Through preliminary in vivo testing in transgenic mice expressing channelrhodopsin (ChR2) we validate that multiple beams of light have an additive effect and increase the response from muscles innervated by the target nerve. Measuring hindlimb flexion increases with increase in number of light sources present. To further characterize this additive effect, a Monte Carlo computer model was generated to simulate a random-walk of photons passing through nerve tissue. The model shows that light beams can aggregate within the nerve, although are limited. When using collimated light, multiple beams converging on the interior region of the nerve cannot result in a higher intensity than outermost layer of tissue nearest a single light source. This model serves as a tool to aid future animal studies by determining light emission parameters, specifically prescribing the need for optically-focused light, when attempting to selectively stimulate regions deep in the interior of a given nerve. Such capability will allow for high spatial resolution of stimulation in peripheral nerves giving finer control of excitation in downstream tissue.

Original languageEnglish (US)
Title of host publication2018 IEEE Life Sciences Conference, LSC 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages154-158
Number of pages5
ISBN (Electronic)9781538667095
DOIs
StatePublished - Dec 10 2018
Event2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada
Duration: Oct 28 2018Oct 30 2018

Other

Other2018 IEEE Life Sciences Conference, LSC 2018
CountryCanada
CityMontreal
Period10/28/1810/30/18

Fingerprint

Optogenetics
nerves
stimulation
Peripheral Nerves
Light
Tissue
muscles
neurons
light sources
Neurons
Light sources
Muscle
nervous system
Opsins
Nerve Tissue
Muscles
random walk
light beams
excitation
light emission

ASJC Scopus subject areas

  • Signal Processing
  • Medicine (miscellaneous)
  • Health Informatics
  • Instrumentation

Cite this

Fritz, N., Gulick, D., & Blain Christen, J. (2018). Computational model of optogenetic stimulation in a peripheral nerve. In 2018 IEEE Life Sciences Conference, LSC 2018 (pp. 154-158). [8572187] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/LSC.2018.8572187

Computational model of optogenetic stimulation in a peripheral nerve. / Fritz, Nicholas; Gulick, Daniel; Blain Christen, Jennifer.

2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 154-158 8572187.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fritz, N, Gulick, D & Blain Christen, J 2018, Computational model of optogenetic stimulation in a peripheral nerve. in 2018 IEEE Life Sciences Conference, LSC 2018., 8572187, Institute of Electrical and Electronics Engineers Inc., pp. 154-158, 2018 IEEE Life Sciences Conference, LSC 2018, Montreal, Canada, 10/28/18. https://doi.org/10.1109/LSC.2018.8572187
Fritz N, Gulick D, Blain Christen J. Computational model of optogenetic stimulation in a peripheral nerve. In 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 154-158. 8572187 https://doi.org/10.1109/LSC.2018.8572187
Fritz, Nicholas ; Gulick, Daniel ; Blain Christen, Jennifer. / Computational model of optogenetic stimulation in a peripheral nerve. 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 154-158
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