Computational analysis and experimental validation of tumor-associated alternative RNA splicing in human cancer

Zhining Wang, H. Shuen Lo, Howard Yang, Sheryl Gere, Ying Hu, Kenneth H. Buetow, Maxwell P. Lee

Research output: Contribution to journalArticle

122 Scopus citations

Abstract

A genome-wide computational screen was performed to identify tumor-associated alternative RNA splicing isoforms. A BLAST algorithm was used to compare 11,014 genes from RefSeq with 3,471,822 human expressed sequence tag sequences. The screen identified 26,258 alternative splicing isoforms of which 845 were significantly associated with human cancer, and 54 were specifically associated with liver cancer. Furthermore, canonical GT-AG splice junctions were used significantly less frequently in the alternative splicing isoforms in tumors. Reverse transcription-PCR experiments confirmed association of the alternative splicing isoforms with tumors. These results suggest that alternative splicing may have potential as a diagnostic marker for cancer.

Original languageEnglish (US)
Pages (from-to)655-657
Number of pages3
JournalCancer Research
Volume63
Issue number3
StatePublished - Feb 1 2003
Externally publishedYes

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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