Computation method to identify differential allelic gene expression and novel imprinted genes

Howard H. Yang, Ying Hu, Michael Edmonson, Kenneth Buetow, Maxwell P. Lee

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Motivation: Genomic imprinting plays an important role in both normal development and diseases. Abnormal imprinting is strongly associated with several human diseases including cancers. Most of the imprinted genes were discovered in the neighborhood of the known imprinted genes. This approach is difficult to extend to analyze the whole genome. We have decided to take a computational approach to systematically search the whole genome for the presence of mono-allelic expressed genes and imprinted genes in human genome. Results: A computational method was developed to identify novel imprinted or mono-allelic genes. Individuals represented in human cDNA libraries were genotyped using Bayesian statistics, and differential expression of polymorphic alleles was identified. A significant reduction in the number of libraries that expressed both alleles, measured by Z-statistics, is a strong indicator for an imprinted or a mono-allelic gene.

Original languageEnglish (US)
Pages (from-to)952-955
Number of pages4
JournalBioinformatics
Volume19
Issue number8
DOIs
StatePublished - May 22 2003
Externally publishedYes

Fingerprint

Differential Expression
Gene expression
Gene Expression
Genes
Gene
Genome
Alleles
Genomic Imprinting
Bayesian Statistics
Human Genome
CDNA
Gene Library
Statistics
Computational Methods
Libraries
Genomics
Cancer
Computational methods
Human
Neoplasms

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Computer Science Applications
  • Computational Theory and Mathematics

Cite this

Computation method to identify differential allelic gene expression and novel imprinted genes. / Yang, Howard H.; Hu, Ying; Edmonson, Michael; Buetow, Kenneth; Lee, Maxwell P.

In: Bioinformatics, Vol. 19, No. 8, 22.05.2003, p. 952-955.

Research output: Contribution to journalArticle

Yang, Howard H. ; Hu, Ying ; Edmonson, Michael ; Buetow, Kenneth ; Lee, Maxwell P. / Computation method to identify differential allelic gene expression and novel imprinted genes. In: Bioinformatics. 2003 ; Vol. 19, No. 8. pp. 952-955.
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