TY - JOUR
T1 - Complex wireframe DNA origami nanostructures with multi-arm junction vertices
AU - Zhang, Fei
AU - Jiang, Shuoxing
AU - Wu, Siyu
AU - Li, Yulin
AU - Mao, Chengde
AU - Liu, Yan
AU - Yan, Hao
N1 - Funding Information:
This research was partly supported by grants to H.Y. and Y.L. from the National Science Foundation (nos. 1360635 and 1334109), the Army Research Office (no. W911NF-12-1-0420) and the National Institutes of Health (no. R01GM104960). H.Y. was supported by the Presidential Strategic Initiative Fund from Arizona State University. The authors thank M. Madjidi for proofreading.
PY - 2015/9/3
Y1 - 2015/9/3
N2 - Structural DNA nanotechnology and the DNA origami technique, in particular, have provided a range of spatially addressable two- and three-dimensional nanostructures. These structures are, however, typically formed of tightly packed parallel helices. The development of wireframe structures should allow the creation of novel designs with unique functionalities, but engineering complex wireframe architectures with arbitrarily designed connections between selected vertices in three-dimensional space remains a challenge. Here, we report a design strategy for fabricating finite-size wireframe DNA nanostructures with high complexity and programmability. In our approach, the vertices are represented by n×4 multi-arm junctions (n=2-10) with controlled angles, and the lines are represented by antiparallel DNA crossover tiles of variable lengths. Scaffold strands are used to integrate the vertices and lines into fully assembled structures displaying intricate architectures. To demonstrate the versatility of the technique, a series of two-dimensional designs including quasi-crystalline patterns and curvilinear arrays or variable curvatures, and three-dimensional designs including a complex snub cube and a reconfigurable Archimedean solid were constructed.
AB - Structural DNA nanotechnology and the DNA origami technique, in particular, have provided a range of spatially addressable two- and three-dimensional nanostructures. These structures are, however, typically formed of tightly packed parallel helices. The development of wireframe structures should allow the creation of novel designs with unique functionalities, but engineering complex wireframe architectures with arbitrarily designed connections between selected vertices in three-dimensional space remains a challenge. Here, we report a design strategy for fabricating finite-size wireframe DNA nanostructures with high complexity and programmability. In our approach, the vertices are represented by n×4 multi-arm junctions (n=2-10) with controlled angles, and the lines are represented by antiparallel DNA crossover tiles of variable lengths. Scaffold strands are used to integrate the vertices and lines into fully assembled structures displaying intricate architectures. To demonstrate the versatility of the technique, a series of two-dimensional designs including quasi-crystalline patterns and curvilinear arrays or variable curvatures, and three-dimensional designs including a complex snub cube and a reconfigurable Archimedean solid were constructed.
UR - http://www.scopus.com/inward/record.url?scp=84941079426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941079426&partnerID=8YFLogxK
U2 - 10.1038/nnano.2015.162
DO - 10.1038/nnano.2015.162
M3 - Article
C2 - 26192207
AN - SCOPUS:84941079426
SN - 1748-3387
VL - 10
SP - 779
EP - 784
JO - Nature nanotechnology
JF - Nature nanotechnology
IS - 9
ER -