Abstract

Using a CH4-based membrane biofilm reactor (MBfR), we studied perchlorate (ClO4-) reduction by a biofilm performing anaerobic methane oxidation coupled to denitrification (ANMO-D). We focused on the effects of nitrate (NO3-) and nitrite (NO2-) surface loadings on ClO4- reduction and on the biofilm community's mechanism for ClO4- reduction. The ANMO-D biofilm reduced up to 5 mg/L of ClO4- to a nondetectable level using CH4 as the only electron donor and carbon source when CH4 delivery was not limiting; NO3- was completely reduced as well when its surface loading was ≥0.32 g N/m2-d. When CH4 delivery was limiting, NO3- inhibited ClO4- reduction by competing for the scarce electron donor. NO2- inhibited ClO4- reduction when its surface loading was ≥0.10 g N/m2-d, probably because of cellular toxicity. Although Archaea were present through all stages, Bacteria dominated the ClO4--reducing ANMO-D biofilm, and gene copies of the particulate methane mono-oxygenase (pMMO) correlated to the increase of respiratory gene copies. These pieces of evidence support that ClO4- reduction by the MBfR biofilm involved chlorite (ClO2-) dismutation to generate the O2 needed as a cosubstrate for the mono-oxygenation of CH4.

Original languageEnglish (US)
Pages (from-to)2341-2349
Number of pages9
JournalEnvironmental Science and Technology
Volume49
Issue number4
DOIs
StatePublished - Feb 17 2015

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

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