Complete Genomic Sequence and Comparative Analysis of the Tumorigenic Poxvirus Yaba Monkey Tumor Virus

Craig R. Brunetti, Hiroko Amano, Yoshiaki Ueda, Jing Qin, Tatsuo Miyamura, Tetsuro Suzuki, Xing Li, John W. Barrett, Douglas McFadden

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The Yatapoxvirus genus of poxviruses is comprised of Yaba monkey tumor virus (YMTV), Tanapox virus, and Yaba-like disease virus (YLDV), which all have the ability to infect primates, including humans. Unlike other poxviruses, YMTV induces formation of focalized histiocytomas upon infection. To gain a greater understanding of the Yatapoxvirus genus and the unique tumor formation properties of YMTV, we sequenced the 134,721-bp genome of YMTV. The genome of YMTV encodes at least 140 open reading frames, all of which are also found as orthologs in the closely related YLDV. However, 13 open reading frames found in YLDV are completely absent from YMTV. Common to both YLDV and YMTV are the unusually large noncoding regions between many open reading frames. To determine whether any of these noncoding regions might be functionally significant, we carried out a comparative analysis between the putative noncoding regions of YMTV and similar noncoding regions from other poxviruses. This approach identified three new gene poxvirus families, defined as orthologs of YMTV23.5L, YMTV28.5L, and YMTV120.5L, which are highly conserved in virtually all poxvirus species. Furthermore, the comparative analysis also revealed a 40-bp nucleotide sequence at approximately 14,700 bases from the left terminus that was 100% identical in the comparable intergene site within members of the Yatapoxvirus, Suipoxvirus, and Capripoxvirus genera and 95% conserved in the Leporipoxvirus genus. This conserved sequence was shown to function as a poxvirus late promoter element in transfected and infected cells, but other functions, such as an involvement in viral replication or packaging, cannot be excluded. Finally, we summarize the predicted immunomodulatory protein repertoire in the Yatapoxvirus genus as a whole.

Original languageEnglish (US)
Pages (from-to)13335-13347
Number of pages13
JournalJournal of Virology
Volume77
Issue number24
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Fingerprint

Yaba monkey tumor virus
Poxviridae
Yatapoxvirus
Sequence Analysis
genomics
Virus Diseases
Open Reading Frames
open reading frames
Suipoxvirus
Leporipoxvirus
Capripoxvirus
Histiocytoma
Genome
genome
conserved sequences
Conserved Sequence
Product Packaging
virus replication
Primates
packaging

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Complete Genomic Sequence and Comparative Analysis of the Tumorigenic Poxvirus Yaba Monkey Tumor Virus. / Brunetti, Craig R.; Amano, Hiroko; Ueda, Yoshiaki; Qin, Jing; Miyamura, Tatsuo; Suzuki, Tetsuro; Li, Xing; Barrett, John W.; McFadden, Douglas.

In: Journal of Virology, Vol. 77, No. 24, 01.12.2003, p. 13335-13347.

Research output: Contribution to journalArticle

Brunetti, CR, Amano, H, Ueda, Y, Qin, J, Miyamura, T, Suzuki, T, Li, X, Barrett, JW & McFadden, D 2003, 'Complete Genomic Sequence and Comparative Analysis of the Tumorigenic Poxvirus Yaba Monkey Tumor Virus', Journal of Virology, vol. 77, no. 24, pp. 13335-13347. https://doi.org/10.1128/JVI.77.24.13335-13347.2003
Brunetti, Craig R. ; Amano, Hiroko ; Ueda, Yoshiaki ; Qin, Jing ; Miyamura, Tatsuo ; Suzuki, Tetsuro ; Li, Xing ; Barrett, John W. ; McFadden, Douglas. / Complete Genomic Sequence and Comparative Analysis of the Tumorigenic Poxvirus Yaba Monkey Tumor Virus. In: Journal of Virology. 2003 ; Vol. 77, No. 24. pp. 13335-13347.
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