Abstract
It is common that phenol and quinoline co-exist in the same industrial wastewater, such as coking wastewater. For both biodegradations, the initial steps are mono-oxygenation reactions, which require two co-substrates: molecular oxygen (O2) and an intercellular electron donor (2H). Competition for O2 and 2H was investigated using a vertical baffled bioreactor (VBBR) with a biofilm acclimated to phenol and quinoline biodegradation. Batch experiments documented mutual inhibition between phenol and quinoline, which competed for O2, 2H, or both during simultaneous biodegradation. Low DO was a limiting factor for phenol and quinoline biodegradations, as both rates slowed significantly for DO ≤ 3 mg/L, compared to DO ≥ 5 mg/L. A DO concentration of 0.5 mg/L led to 89% and 65% slower removal kinetics for phenol and quinoline, respectively. Although adding succinate as an exogenous electron donor was able to alleviate competition when the DO was 4 ∼ 5 mg/L, it had no benefit for a DO ≤ 3 mg/L. Thus, significant DO limitation could not be overcome by addition of more donor. The results imply that a strategy that involves adding or creating an exogenous electron donor may be effective only when DO is not significantly rate limiting for the initial oxygenation reactions.
| Original language | English (US) |
|---|---|
| Journal | Process Biochemistry |
| DOIs | |
| State | Accepted/In press - Jan 1 2018 |
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Keywords
- Biodegradation
- Intracellular electron donors
- Molecular oxygen
- Phenol
- Quinoline
ASJC Scopus subject areas
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
Cite this
Competition for molecular oxygen and electron donor between phenol and quinoline during their simultaneous biodegradation. / Zou, Shasha; Zhang, Bingbing; Yan, Ning; Zhang, Chenyuan; Xu, Hua; Zhang, Yongming; Rittmann, Bruce.
In: Process Biochemistry, 01.01.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Competition for molecular oxygen and electron donor between phenol and quinoline during their simultaneous biodegradation
AU - Zou, Shasha
AU - Zhang, Bingbing
AU - Yan, Ning
AU - Zhang, Chenyuan
AU - Xu, Hua
AU - Zhang, Yongming
AU - Rittmann, Bruce
PY - 2018/1/1
Y1 - 2018/1/1
N2 - It is common that phenol and quinoline co-exist in the same industrial wastewater, such as coking wastewater. For both biodegradations, the initial steps are mono-oxygenation reactions, which require two co-substrates: molecular oxygen (O2) and an intercellular electron donor (2H). Competition for O2 and 2H was investigated using a vertical baffled bioreactor (VBBR) with a biofilm acclimated to phenol and quinoline biodegradation. Batch experiments documented mutual inhibition between phenol and quinoline, which competed for O2, 2H, or both during simultaneous biodegradation. Low DO was a limiting factor for phenol and quinoline biodegradations, as both rates slowed significantly for DO ≤ 3 mg/L, compared to DO ≥ 5 mg/L. A DO concentration of 0.5 mg/L led to 89% and 65% slower removal kinetics for phenol and quinoline, respectively. Although adding succinate as an exogenous electron donor was able to alleviate competition when the DO was 4 ∼ 5 mg/L, it had no benefit for a DO ≤ 3 mg/L. Thus, significant DO limitation could not be overcome by addition of more donor. The results imply that a strategy that involves adding or creating an exogenous electron donor may be effective only when DO is not significantly rate limiting for the initial oxygenation reactions.
AB - It is common that phenol and quinoline co-exist in the same industrial wastewater, such as coking wastewater. For both biodegradations, the initial steps are mono-oxygenation reactions, which require two co-substrates: molecular oxygen (O2) and an intercellular electron donor (2H). Competition for O2 and 2H was investigated using a vertical baffled bioreactor (VBBR) with a biofilm acclimated to phenol and quinoline biodegradation. Batch experiments documented mutual inhibition between phenol and quinoline, which competed for O2, 2H, or both during simultaneous biodegradation. Low DO was a limiting factor for phenol and quinoline biodegradations, as both rates slowed significantly for DO ≤ 3 mg/L, compared to DO ≥ 5 mg/L. A DO concentration of 0.5 mg/L led to 89% and 65% slower removal kinetics for phenol and quinoline, respectively. Although adding succinate as an exogenous electron donor was able to alleviate competition when the DO was 4 ∼ 5 mg/L, it had no benefit for a DO ≤ 3 mg/L. Thus, significant DO limitation could not be overcome by addition of more donor. The results imply that a strategy that involves adding or creating an exogenous electron donor may be effective only when DO is not significantly rate limiting for the initial oxygenation reactions.
KW - Biodegradation
KW - Intracellular electron donors
KW - Molecular oxygen
KW - Phenol
KW - Quinoline
UR - http://www.scopus.com/inward/record.url?scp=85046643704&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046643704&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2018.04.015
DO - 10.1016/j.procbio.2018.04.015
M3 - Article
AN - SCOPUS:85046643704
JO - Process Biochemistry
JF - Process Biochemistry
SN - 0032-9592
ER -