Comparison of two serum bactericidal assays for Neisseria gonorrhoeae

Robin K. Pettit, Joan C. Szuba, Ralph C. Judd

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Abstract

Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested. When data for all of the strains were compared, no correlation was found between the dilution plate and microtiter bactericidal assays. Finally, we found that the bactericidal capacities of intact and complement-depleted human sera very similar when assessed by microtiter methods, suggesting a non-complement-mediated serum killing mechanism.

Original languageEnglish (US)
Pages (from-to)15-22
Number of pages8
JournalJournal of Immunological Methods
Volume129
Issue number1
DOIs
StatePublished - May 8 1990
Externally publishedYes

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Keywords

  • Bactericidal assay
  • Neisseria gonorrhoeae
  • Normal human serum

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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