Comparison of two serum bactericidal assays for Neisseria gonorrhoeae

Robin K. Pettit; Joan C. Szuba; Ralph C. Judd

doi:10.1016/0022-1759(90)90415-R

Comparison of two serum bactericidal assays for Neisseria gonorrhoeae

Robin K. Pettit, Joan C. Szuba, Ralph C. Judd

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested. When data for all of the strains were compared, no correlation was found between the dilution plate and microtiter bactericidal assays. Finally, we found that the bactericidal capacities of intact and complement-depleted human sera very similar when assessed by microtiter methods, suggesting a non-complement-mediated serum killing mechanism.

Original language	English (US)
Pages (from-to)	15-22
Number of pages	8
Journal	Journal of Immunological Methods
Volume	129
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(90)90415-R
State	Published - May 8 1990
Externally published	Yes

Keywords

Bactericidal assay
Neisseria gonorrhoeae
Normal human serum

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0022-1759(90)90415-R

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title = "Comparison of two serum bactericidal assays for Neisseria gonorrhoeae",

abstract = "Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested. When data for all of the strains were compared, no correlation was found between the dilution plate and microtiter bactericidal assays. Finally, we found that the bactericidal capacities of intact and complement-depleted human sera very similar when assessed by microtiter methods, suggesting a non-complement-mediated serum killing mechanism.",

keywords = "Bactericidal assay, Neisseria gonorrhoeae, Normal human serum",

author = "Pettit, {Robin K.} and Szuba, {Joan C.} and Judd, {Ralph C.}",

note = "Funding Information: This work was sponsoredb y Public Health ServiceG rants R01 AI21236 and K04 AI00834 and Universityo f MontanaR esearchG rant1117. We thank Drs. Kenneth Dial and Willard Granath for their assistancein the statistical analysesa nd Drs. Keith Joiner, Brad McMullin, William Shafer,a nd John Swansonf or providing bacteriasl trainsa nd serumr eagents.",

year = "1990",

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doi = "10.1016/0022-1759(90)90415-R",

language = "English (US)",

volume = "129",

pages = "15--22",

journal = "Journal of Immunological Methods",

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T1 - Comparison of two serum bactericidal assays for Neisseria gonorrhoeae

AU - Pettit, Robin K.

AU - Szuba, Joan C.

AU - Judd, Ralph C.

N1 - Funding Information: This work was sponsoredb y Public Health ServiceG rants R01 AI21236 and K04 AI00834 and Universityo f MontanaR esearchG rant1117. We thank Drs. Kenneth Dial and Willard Granath for their assistancein the statistical analysesa nd Drs. Keith Joiner, Brad McMullin, William Shafer,a nd John Swansonf or providing bacteriasl trainsa nd serumr eagents.

PY - 1990/5/8

Y1 - 1990/5/8

N2 - Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested. When data for all of the strains were compared, no correlation was found between the dilution plate and microtiter bactericidal assays. Finally, we found that the bactericidal capacities of intact and complement-depleted human sera very similar when assessed by microtiter methods, suggesting a non-complement-mediated serum killing mechanism.

AB - Pooled normal human serum killing of 14 strains of Neisseria gonorrhoeae was assessed by dilution plate and microtiter methods. In both assays, the strains presented a spectrum of sensitivity to the serum. In the dilution plate assay, results with two different concentrations of human serum were similar for most, but not all of the strains tested. When data for all of the strains were compared, no correlation was found between the dilution plate and microtiter bactericidal assays. Finally, we found that the bactericidal capacities of intact and complement-depleted human sera very similar when assessed by microtiter methods, suggesting a non-complement-mediated serum killing mechanism.

KW - Bactericidal assay

KW - Neisseria gonorrhoeae

KW - Normal human serum

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U2 - 10.1016/0022-1759(90)90415-R

DO - 10.1016/0022-1759(90)90415-R

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JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

Comparison of two serum bactericidal assays for Neisseria gonorrhoeae

Abstract

Keywords

ASJC Scopus subject areas

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