Comparison of RNAi knockdown effect of tyramine receptor 1 induced by dsRNA and siRNA in brains of the honey bee, Apis mellifera

Xiaojiao Guo, Ying Wang, Irina Sinakevitch, Hong Lei, Brian Smith

Research output: Contribution to journalArticle

Abstract

RNA interference (RNAi) is a powerful tool for artificially manipulating gene expression in diverse organisms. In the honey bee, Apis mellifera, both long double stranded RNA (dsRNA) and small interference RNA (siRNA) have been successfully used to reduce targeted gene expression and induce specific phenotypes. However, whether dsRNA and siRNA have different effects and efficiencies in gene silencing has never been investigated in honey bees. Thus, we tested the effect of dsRNA and siRNA on the tyramine receptor 1 (tyr1), which encodes a receptor of neurotransmitter tyramine, in honey bee brains at mRNA and protein levels over time. We found that both dsRNA and siRNA achieved successful gene knockdown. The siRNA mixes affected tyr1 gene expression faster than dsRNA, and the duration of the knockdown between dsRNA and siRNA varied. We also found that the turnover rate of TYR1 protein was relatively fast, which is consistent with its role as a neurotransmitter receptor. Our study reveals the different efficiencies of dsRNA and siRNA in honey bee brains. We show that consideration of the gene regions targeted by RNAi, prior screening for RNAi molecules and combing siRNAs are important strategies to enhance RNAi efficiency.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalJournal of Insect Physiology
Volume111
DOIs
StatePublished - Nov 1 2018

Fingerprint

Honey
Double-Stranded RNA
tyramine
Bees
double-stranded RNA
RNA Interference
RNA interference
Apis mellifera
honey bees
RNA
brain
receptors
Brain
Neurotransmitter Receptor
Gene Expression
gene expression
tyramine receptor
Gene Knockdown Techniques
Gene Silencing
gene silencing

Keywords

  • Brain
  • Double-stranded RNA (dsRNA)
  • Honey bee
  • mRNA
  • Protein
  • RNA interference (RNAi)
  • Small interference RNA (siRNA)
  • Tyramine receptor 1 (tyr1)

ASJC Scopus subject areas

  • Physiology
  • Insect Science

Cite this

Comparison of RNAi knockdown effect of tyramine receptor 1 induced by dsRNA and siRNA in brains of the honey bee, Apis mellifera. / Guo, Xiaojiao; Wang, Ying; Sinakevitch, Irina; Lei, Hong; Smith, Brian.

In: Journal of Insect Physiology, Vol. 111, 01.11.2018, p. 47-52.

Research output: Contribution to journalArticle

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