Comparison of properties between NIPAAm-based simultaneously physically and chemically gelling polymer systems for use in vivo

Hanin H. Bearat, Bae Hoon Lee, Brent Vernon

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In this work, a comparison between two different physical-chemical gels, poly(NIPAAm-co-cysteamine) with poly(NIPAAm-co-cysteamine-vinylsulfone) and poly(NIPAAm-co-cysteamine) with poly(NIPAAm-co-HEMA-acrylate), is made. These hydrogels undergo gelation via dual mechanisms: temperature sensitivity (physical gelation) and chemical crosslinking (chemical gelation). The advantages of using both gelation mechanisms are to reduce the creep experienced by purely physical gels and to increase the elastic modulus of purely chemical gels. Here, the physical-chemical gels were synthesized and characterized for their chemical, structural, thermal, mechanical and morphological properties. The gels were also tested for their gelation kinetics, swelling, degradation and cytotoxicity. The copolymers were successfully synthesized and their phase transition temperatures fall in a feasible range (29-34°C) for use in vivo. With rheology, it was shown that use of simultaneous physical and chemical gelation resulted in improved properties, with increased elastic moduli and reduced frequency dependence. The rates of reaction of thiols to vinyls differ between the two systems, demonstrating a greater effect of chemical gelation in one gelling system over the other, due to the faster rate of thiols consumed into reaction. The morphology of the gels proved to be quite different when analyzed by scanning electron microscopy, showing differences in swelling behaviors. Cell studies illustrated good growth of cells exposed to the gels. Both hydrogels, although possessing slight differences, demonstrate the capability of being injected in vivo for use as embolic agents for occlusion of aneurysms.

Original languageEnglish (US)
Pages (from-to)3629-3642
Number of pages14
JournalActa Biomaterialia
Volume8
Issue number10
DOIs
StatePublished - Oct 2012

Fingerprint

Gelation
Polymers
Gels
Hydrogels
Elastic Modulus
Sulfhydryl Compounds
Swelling
Elastic moduli
Rheology
Transition Temperature
Phase Transition
Cytotoxicity
Crosslinking
Electron Scanning Microscopy
Superconducting transition temperature
Aneurysm
Creep
Copolymers
Hot Temperature
Phase transitions

Keywords

  • Hydrogel
  • In situ gelling polymer system
  • Michael-type addition reaction
  • Poly(NIPAAm)
  • Thermoresponsive

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Comparison of properties between NIPAAm-based simultaneously physically and chemically gelling polymer systems for use in vivo. / Bearat, Hanin H.; Lee, Bae Hoon; Vernon, Brent.

In: Acta Biomaterialia, Vol. 8, No. 10, 10.2012, p. 3629-3642.

Research output: Contribution to journalArticle

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