Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization

C. E. Enriquez, Morteza Abbaszadegan, I. L. Pepper, K. J. Richardson, A. B. Margolin, C. P. Gerba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalWater Science and Technology
Volume27
Issue number3-4
StatePublished - 1993

Fingerprint

Nucleic acids
nucleic acid
Cell culture
well water
assay
Assays
Water
Tissue culture
virus
Viruses
water
probe
phosphate
Phosphates
Genes
gene
infectivity
detection
comparison
filter

Keywords

  • Nucleic acid hybridization
  • Poliovirus
  • Viral detection

ASJC Scopus subject areas

  • Water Science and Technology

Cite this

Enriquez, C. E., Abbaszadegan, M., Pepper, I. L., Richardson, K. J., Margolin, A. B., & Gerba, C. P. (1993). Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization. Water Science and Technology, 27(3-4), 315-319.

Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization. / Enriquez, C. E.; Abbaszadegan, Morteza; Pepper, I. L.; Richardson, K. J.; Margolin, A. B.; Gerba, C. P.

In: Water Science and Technology, Vol. 27, No. 3-4, 1993, p. 315-319.

Research output: Contribution to journalArticle

Enriquez, CE, Abbaszadegan, M, Pepper, IL, Richardson, KJ, Margolin, AB & Gerba, CP 1993, 'Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization', Water Science and Technology, vol. 27, no. 3-4, pp. 315-319.
Enriquez, C. E. ; Abbaszadegan, Morteza ; Pepper, I. L. ; Richardson, K. J. ; Margolin, A. B. ; Gerba, C. P. / Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization. In: Water Science and Technology. 1993 ; Vol. 27, No. 3-4. pp. 315-319.
@article{2b58fc72b2e54ca28893331dde3aeeb2,
title = "Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization",
abstract = "The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.",
keywords = "Nucleic acid hybridization, Poliovirus, Viral detection",
author = "Enriquez, {C. E.} and Morteza Abbaszadegan and Pepper, {I. L.} and Richardson, {K. J.} and Margolin, {A. B.} and Gerba, {C. P.}",
year = "1993",
language = "English (US)",
volume = "27",
pages = "315--319",
journal = "Water Science and Technology",
issn = "0273-1223",
publisher = "IWA Publishing",
number = "3-4",

}

TY - JOUR

T1 - Comparison of poliovirus detection in water by cell culture and nucleic acid hybridization

AU - Enriquez, C. E.

AU - Abbaszadegan, Morteza

AU - Pepper, I. L.

AU - Richardson, K. J.

AU - Margolin, A. B.

AU - Gerba, C. P.

PY - 1993

Y1 - 1993

N2 - The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.

AB - The nucleic acid hybridization technique has been used to detect viral nucleic acid in environmental water samples. This type of assay, in contrast with tissue culture assays, may not distinguish between viable and non-viable viruses. We evaluated, by comparison with tissue culture infectivity assay (plaque forming method), the ability of the gene probe assay to detect viable poliovirus 1 (LSc) in well water, autoclaved well water, filter-sterilized well water and autoclaved phosphate buffered saline kept at 37° C and 15° C for 75 days, and in dechlorinated tapwater held at room temperature. A gradual decline in numbers of poliovirus was observed in all of the samples by cell culture assay. With the exception of autoclaved well water and phosphate buffer samples, a parallel decline in virus detectable by gene probe occurred in all other water samples.

KW - Nucleic acid hybridization

KW - Poliovirus

KW - Viral detection

UR - http://www.scopus.com/inward/record.url?scp=0027208010&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027208010&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 315

EP - 319

JO - Water Science and Technology

JF - Water Science and Technology

SN - 0273-1223

IS - 3-4

ER -