Comparison of mechanisms involved in impaired vascular reactivity between high sucrose and high fat diets in rats

Karen Sweazea, Mateja Lekic, Benjimen R. Walker

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: To determine the effects of high sucrose diets on vascular reactivity. We hypothesized that similar to high fat diets (HFD), HSD feeding would lead to increased adiposity resulting in inflammation and oxidative stress-mediated impairment of vasodilation. Methods. Male Sprague-Dawley rats were fed control chow (Chow), HSD or HFD diets for 6 weeks. The role of inflammation and oxidative stress on impaired vasodilation were assessed in isolated mesenteric arterioles. Results: HSD and HFD induced increased adiposity, oxidative stress and inflammation. HFD rats developed fasting hyperglycemia. Both HSD and HFD rats developed impaired glucose tolerance and hyperleptinemia. Nitric oxide (NO)-mediated vasodilation was significantly attenuated in both HSD and HFD rats but was normalized by treatment with antioxidants or anti-inflammatory drugs. Endothelial NO synthase (eNOS) protein expression was not affected by diet. Sensitivity to NO was reduced since NOS inhibition attenuated vasodilation in Chow rats but did not further impair vasodilation in HSD or HFD rats. Likewise, responsiveness to a NO donor was attenuated in both experimental groups. Conclusions: Oxidative stress diminishes vasodilatory responsiveness in HSD and HFD rats through ROS-mediated scavenging of NO and decreased smooth muscle sensitivity to NO. Inflammation also plays a significant role in the impaired vasodilation.

Original languageEnglish (US)
Article number48
JournalNutrition and Metabolism
Volume7
DOIs
StatePublished - 2010

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High Fat Diet
Blood Vessels
Sucrose
Vasodilation
Nitric Oxide
Oxidative Stress
Inflammation
Adiposity
Diet
Glucose Intolerance
Nitric Oxide Donors
Nitric Oxide Synthase Type III
Arterioles
Hyperglycemia
Smooth Muscle
Sprague Dawley Rats
Fasting
Anti-Inflammatory Agents
Antioxidants
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics
  • Endocrinology, Diabetes and Metabolism

Cite this

Comparison of mechanisms involved in impaired vascular reactivity between high sucrose and high fat diets in rats. / Sweazea, Karen; Lekic, Mateja; Walker, Benjimen R.

In: Nutrition and Metabolism, Vol. 7, 48, 2010.

Research output: Contribution to journalArticle

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