TY - JOUR
T1 - Comparative Transcriptomics of Early Meiosis in Arabidopsis and Maize
AU - Dukowic-Schulze, Stefanie
AU - Harris, Anthony
AU - Li, Junhua
AU - Sundararajan, Anitha
AU - Mudge, Joann
AU - Retzel, Ernest F.
AU - Pawlowski, Wojciech P.
AU - Chen, Changbin
N1 - Funding Information:
We thank T. Li and D. McDowell for plant care, and R. Meissner and D. Brinkmann for facility support. This work was supported by the National Science Foundation of USA (IOS: 1025881 ) to W.P. and C.C., and by funds from the Biotechnology Research and Development Corporation of USA to C.C.
PY - 2014/3/20
Y1 - 2014/3/20
N2 - Though sexually reproductive plants share the same principle and most processes in meiosis, there are distinct features detectable. To address the similarities and differences of early meiosis transcriptomes from the dicot model system Arabidopsis and monocot model system maize, we performed comparative analyses of RNA-seq data of isolated meiocytes, anthers and seedlings from both species separately and via orthologous genes. Overall gene expression showed similarities, such as an increased number of reads mapping to unannotated features, and differences, such as the amount of differentially expressed genes. We detected major similarities and differences in functional annotations of genes up-regulated in meiocytes, which point to conserved features as well as unique features. Transcriptional regulation seems to be quite similar in Arabidopsis and maize, and we could reveal known and novel transcription factors and cis-regulatory elements acting in early meiosis. Taken together, meiosis between Arabidopsis and maize is conserved in many ways, but displays key distinctions that lie in the patterns of gene expression.
AB - Though sexually reproductive plants share the same principle and most processes in meiosis, there are distinct features detectable. To address the similarities and differences of early meiosis transcriptomes from the dicot model system Arabidopsis and monocot model system maize, we performed comparative analyses of RNA-seq data of isolated meiocytes, anthers and seedlings from both species separately and via orthologous genes. Overall gene expression showed similarities, such as an increased number of reads mapping to unannotated features, and differences, such as the amount of differentially expressed genes. We detected major similarities and differences in functional annotations of genes up-regulated in meiocytes, which point to conserved features as well as unique features. Transcriptional regulation seems to be quite similar in Arabidopsis and maize, and we could reveal known and novel transcription factors and cis-regulatory elements acting in early meiosis. Taken together, meiosis between Arabidopsis and maize is conserved in many ways, but displays key distinctions that lie in the patterns of gene expression.
KW - Arabidopsis
KW - Maize
KW - Meiocytes
KW - Meiosis
KW - RNA-seq
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=84896316569&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84896316569&partnerID=8YFLogxK
U2 - 10.1016/j.jgg.2013.11.007
DO - 10.1016/j.jgg.2013.11.007
M3 - Article
C2 - 24656234
AN - SCOPUS:84896316569
SN - 1673-8527
VL - 41
SP - 139
EP - 152
JO - Journal of Genetics and Genomics
JF - Journal of Genetics and Genomics
IS - 3
ER -