Comparative RNA-Seq analysis reveals pervasive tissue-specific alternative polyadenylation in Caenorhabditis elegans intestine and muscles

Stephen M. Blazie, Cody Babb, Henry Wilky, Alan Rawls, Jin G. Park, Marco Mangone

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Tissue-specific RNA plasticity broadly impacts the development, tissue identity and adaptability of all organisms, but changes in composition, expression levels and its impact on gene regulation in different somatic tissues are largely unknown. Here we developed a new method, polyA-tagging and sequencing (PAT-Seq) to isolate high-quality tissue-specific mRNA from Caenorhabditis elegans intestine, pharynx and body muscle tissues and study changes in their tissue-specific transcriptomes and 3'UTRomes. Results: We have identified thousands of novel genes and isoforms differentially expressed between these three tissues. The intestine transcriptome is expansive, expressing over 30% of C. elegans mRNAs, while muscle transcriptomes are smaller but contain characteristic unique gene signatures. Active promoter regions in all three tissues reveal both known and novel enriched tissue-specific elements, along with putative transcription factors, suggesting novel tissue-specific modes of transcription initiation. We have precisely mapped approximately 20,000 tissue-specific polyadenylation sites and discovered that about 30% of transcripts in somatic cells use alternative polyadenylation in a tissue-specific manner, with their 3'UTR isoforms significantly enriched with microRNA targets. Conclusions: For the first time, PAT-Seq allowed us to directly study tissue specific gene expression changes in an in vivo setting and compare these changes between three somatic tissues from the same organism at single-base resolution within the same experiment. We pinpoint precise tissue-specific transcriptome rearrangements and for the first time link tissue-specific alternative polyadenylation to miRNA regulation, suggesting novel and unexplored tissue-specific post-transcriptional regulatory networks in somatic cells.

Original languageEnglish (US)
Article number4
JournalBMC Biology
Volume13
Issue number1
DOIs
StatePublished - Jan 20 2015

Fingerprint

Polyadenylation
Caenorhabditis elegans
Intestines
Muscle
RNA
muscle
intestines
Tissue
Muscles
muscles
Transcriptome
transcriptome
tissues
analysis
tissue
tagging
MicroRNAs
microRNA
somatic cells
Gene expression

Keywords

  • 3'UTR
  • Elegans
  • miRNAs
  • mRNA-tagging
  • PAT-Seq
  • Promoter
  • RNA-Seq
  • Tissue-specific mRNA
  • Transcription
  • Transcriptome

ASJC Scopus subject areas

  • Cell Biology
  • Ecology, Evolution, Behavior and Systematics
  • Plant Science
  • Developmental Biology
  • Structural Biology
  • Biotechnology
  • Physiology
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Comparative RNA-Seq analysis reveals pervasive tissue-specific alternative polyadenylation in Caenorhabditis elegans intestine and muscles. / Blazie, Stephen M.; Babb, Cody; Wilky, Henry; Rawls, Alan; Park, Jin G.; Mangone, Marco.

In: BMC Biology, Vol. 13, No. 1, 4, 20.01.2015.

Research output: Contribution to journalArticle

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