Comparative phenotypic analyses of human plasma and urinary retinol binding protein using mass spectrometric immunoassay

Urban A. Kiernan, Kemmons A. Tubbs, Dobrin Nedelkov, Eric E. Niederkofler, Randall W. Nelson

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Mass spectrometric immunoassay (MSIA) is a proteomics technology that combines the selectivity of affinity capture with the sensitivity and resolution of mass spectrometric detection. This unique approach allows for intact protein identification therefore is readily capable of discriminating between protein variants, i.e., mutations, posttranslational modifications, and truncations. In this work, MSIA is used in the comparative analyses of retinol binding protein (RBP) from the plasma and urine of a small study population. Detailed RBP profiles were obtained from both biological fluids, resulting in the identification of several catabolic RBP products (present in urine) that have not been previously reported. In addition, comparative analysis of urine samples taken from healthy and renally impaired individuals revealed different breakdown profiles. These results illustrate the use of MSIA for the rapid, sensitive, and accurate profiling of RBP both within and between individuals.

Original languageEnglish (US)
Pages (from-to)401-405
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume297
Issue number2
DOIs
StatePublished - Nov 6 2002

Keywords

  • Humans
  • MALDI-TOF MS
  • Posttranslational modifications
  • Retinol binding protein
  • Urine protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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