Comparative interactomics: Analysis of Arabidopsis 14-3-3 complexes reveals highly conserved 14-3-3 interactions between humans and plants

Anna Lisa Paul, Li Liu, Scott McClung, Beth Laughner, Sixue Chen, Robert J. Ferl

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

As a first step in the broad characterization of plant 14-3-3 multiprotein complexes in vivo, stringent and specific antibody affinity purification was used to capture 14-3-3s together with their interacting proteins from extracts of Arabidopsis cell suspension cultures. Approximately 120 proteins were identified as potential in vivo 14-3-3 interacting proteins by mass spectrometry of the recovered complexes. Comparison of the proteins in this data set with the 14-3-3 interacting proteins from a similar study in human embryonic kidney cell cultures revealed eight interacting proteins that likely represent reasonably abundant, fundamental 14-3-3 interaction complexes that are highly conserved across all eukaryotes. The Arabidopsis 14-3-3 interaction data set was also compared to a yeast in vivo 14-3-3 interaction data set. Four 14-3-3 interacting proteins are conserved in yeast, humans, and Arabidopsis. Comparisons of the data sets based on biochemical function revealed many additional similarities in the human and Arabidopsis data sets that represent conserved functional interactions, while also leaving many proteins uniquely identified in either Arabidopsis or human cells. In particular, the Arabidopsis interaction data set is enriched for proteins involved in metabolism.

Original languageEnglish (US)
Pages (from-to)1913-1924
Number of pages12
JournalJournal of Proteome Research
Volume8
Issue number4
DOIs
StatePublished - Apr 3 2009
Externally publishedYes

Keywords

  • 14-3-3
  • Interactome
  • Multiprotein complexes
  • Plant
  • Primary metabolism
  • Proteome

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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