Comparative genome analysis of the high pathogenicity salmonella typhimurium strain UK-1

Yingqin Luo, Qingke Kong, Jiseon Yang, Arindam Mitra, Greg Golden, Soo Young Wanda, Kenneth L. Roland, Roderick V. Jensen, Peter B. Ernst, Roy Curtiss

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Salmonella enterica serovar Typhimurium, a gram-negative facultative rod-shaped bacterium causing salmonellosis and foodborne disease, is one of the most common isolated Salmonella serovars in both developed and developing nations. Several S. Typhimurium genomes have been completed and many more genome-sequencing projects are underway. Comparative genome analysis of the multiple strains leads to a better understanding of the evolution of S. Typhimurium and its pathogenesis. S. Typhimurium strain UK-1 (belongs to phage type 1) is highly virulent when orally administered to mice and chickens and efficiently colonizes lymphoid tissues of these species. These characteristics make this strain a good choice for use in vaccine development. In fact, UK-1 has been used as the parent strain for a number of nonrecombinant and recombinant vaccine strains, including several commercial vaccines for poultry. In this study, we conducted a thorough comparative genome analysis of the UK-1 strain with other S. Typhimurium strains and examined the phenotypic impact of several genomic differences. Whole genomic comparison highlights an extremely close relationship between the UK-1 strain and other S. Typhimurium strains; however, many interesting genetic and genomic variations specific to UK-1 were explored. In particular, the deletion of a UK-1-specific gene that is highly similar to the gene encoding the T3SS effector protein NleC exhibited a significant decrease in oral virulence in BALB/c mice. The complete genetic complements in UK-1, especially those elements that contribute to virulence or aid in determining the diversity within bacterial species, provide key information in evaluating the functional characterization of important genetic determinants and for development of vaccines.

Original languageEnglish (US)
Article numbere40645
JournalPLoS One
Volume7
Issue number7
DOIs
StatePublished - Jul 6 2012

Fingerprint

Salmonella
Salmonella typhimurium
Salmonella Typhimurium
Virulence
pathogenicity
Genes
Genome
genome
Vaccines
vaccine development
genomics
Poultry
virulence
Synthetic Vaccines
Bacteriophages
Gene encoding
Foodborne Diseases
Salmonella enterica
Salmonella Infections
Lymphoid Tissue

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Luo, Y., Kong, Q., Yang, J., Mitra, A., Golden, G., Wanda, S. Y., ... Curtiss, R. (2012). Comparative genome analysis of the high pathogenicity salmonella typhimurium strain UK-1. PLoS One, 7(7), [e40645]. https://doi.org/10.1371/journal.pone.0040645

Comparative genome analysis of the high pathogenicity salmonella typhimurium strain UK-1. / Luo, Yingqin; Kong, Qingke; Yang, Jiseon; Mitra, Arindam; Golden, Greg; Wanda, Soo Young; Roland, Kenneth L.; Jensen, Roderick V.; Ernst, Peter B.; Curtiss, Roy.

In: PLoS One, Vol. 7, No. 7, e40645, 06.07.2012.

Research output: Contribution to journalArticle

Luo, Y, Kong, Q, Yang, J, Mitra, A, Golden, G, Wanda, SY, Roland, KL, Jensen, RV, Ernst, PB & Curtiss, R 2012, 'Comparative genome analysis of the high pathogenicity salmonella typhimurium strain UK-1', PLoS One, vol. 7, no. 7, e40645. https://doi.org/10.1371/journal.pone.0040645
Luo, Yingqin ; Kong, Qingke ; Yang, Jiseon ; Mitra, Arindam ; Golden, Greg ; Wanda, Soo Young ; Roland, Kenneth L. ; Jensen, Roderick V. ; Ernst, Peter B. ; Curtiss, Roy. / Comparative genome analysis of the high pathogenicity salmonella typhimurium strain UK-1. In: PLoS One. 2012 ; Vol. 7, No. 7.
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