Comparative analysis of eukaryotic cell-Free expression systems

Emily M. Hartsough, Pankti Shah, Andrew C. Larsen, John C. Chaput

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cell-free protein synthesis (CFPS) allows researchers to rapidly generate functional proteins independent of cell culture. Although advances in eukaryotic lysates have increased the amount of protein that can be produced, the nuances of different translation systems lead to variability in protein production. To help overcome this problem, we have compared the relative yield and template requirements for three commonly used commercial cell-free translation systems: wheat germ extract (WGE), rabbit reticulocyte lysate (RRL), and HeLa cell lysate (HCL). Our results provide a general guide for researchers interested in using cell-free translation to generate recombinant protein for biomedical applications.

Original languageEnglish (US)
Pages (from-to)149-151
Number of pages3
JournalBioTechniques
Volume59
Issue number3
DOIs
StatePublished - Sep 1 2015

Fingerprint

Cell-Free System
Eukaryotic Cells
Proteins
Research Personnel
Reticulocytes
HeLa Cells
Cell culture
Recombinant Proteins
Triticum
Cell Culture Techniques
Rabbits

Keywords

  • Cell-free translation
  • Recombinant protein synthesis
  • Translation enhancing elements

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Cite this

Hartsough, E. M., Shah, P., Larsen, A. C., & Chaput, J. C. (2015). Comparative analysis of eukaryotic cell-Free expression systems. BioTechniques, 59(3), 149-151. https://doi.org/10.2144/000114327

Comparative analysis of eukaryotic cell-Free expression systems. / Hartsough, Emily M.; Shah, Pankti; Larsen, Andrew C.; Chaput, John C.

In: BioTechniques, Vol. 59, No. 3, 01.09.2015, p. 149-151.

Research output: Contribution to journalArticle

Hartsough, EM, Shah, P, Larsen, AC & Chaput, JC 2015, 'Comparative analysis of eukaryotic cell-Free expression systems', BioTechniques, vol. 59, no. 3, pp. 149-151. https://doi.org/10.2144/000114327
Hartsough EM, Shah P, Larsen AC, Chaput JC. Comparative analysis of eukaryotic cell-Free expression systems. BioTechniques. 2015 Sep 1;59(3):149-151. https://doi.org/10.2144/000114327
Hartsough, Emily M. ; Shah, Pankti ; Larsen, Andrew C. ; Chaput, John C. / Comparative analysis of eukaryotic cell-Free expression systems. In: BioTechniques. 2015 ; Vol. 59, No. 3. pp. 149-151.
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