Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis

Yunho Gwak, Yong Sic Hwang, Baobei Wang, Minju Kim, Jooyeon Jeong, Choul Gyun Lee, Qiang Hu, Danxiang Han, Eonseon Jin

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Haematococcus pluvialis cells predominantly remain in the macrozooid stage under favourable environmental conditions but are rapidly differentiated into haematocysts upon exposure to various environmental stresses. Haematocysts are characterized by massive accumulations of astaxanthin sequestered in cytosolic oil globules. Lipidomic analyses revealed that synthesis of the storage lipid triacylglycerol (TAG) was substantially stimulated under high irradiance. Simultaneously, remodelling of membrane glycerolipids occurred as a result of dramatic reductions in chloroplast membrane glycolipids but remained unchanged or declined slightly in extraplastidic membrane glycerolipids. De novo assembly of transcriptomes revealed the genomic and metabolic features of this unsequenced microalga. Comparative transcriptomic analysis showed that so-called resting cells (haematocysts) may be more active than fast-growing vegetative cells (macrozooids) regarding metabolic pathways and functions. Comparative transcriptomic analyses of astaxanthin biosynthesis suggested that the non-mevalonate pathway mediated the synthesis of isopentenyl diphosphate, as the majority of genes involved in subsequent astaxanthin biosynthesis were substantially up-regulated under high irradiance, with the genes encoding phytoene synthase, phytoene desaturase, and β-carotene hydroxylase identified as the most prominent regulatory components. Accumulation of TAG under high irradiance was attributed to moderate up-regulation of de novo fatty acid biosynthesis at the gene level as well as to moderate elevation of the TAG assembly pathways. Additionally, inferred from transcriptomic differentiation, an increase in reactive oxygen species (ROS) scavenging activity, a decrease in ROS production, and the relaxation of over-reduction of the photosynthetic electron transport chain will work together to protect against photooxidative stress in H. pluvialis under high irradiance.

Original languageEnglish (US)
Pages (from-to)4317-4334
Number of pages18
JournalJournal of Experimental Botany
Volume65
Issue number15
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Haematococcus pluvialis
astaxanthin
Gene Expression Profiling
transcriptomics
transcriptome
Triglycerides
triacylglycerols
biosynthesis
Membranes
reactive oxygen species
Reactive Oxygen Species
phytoene synthase
Genes
synthesis
genes
glycolipids
vegetative cells
Glycolipids
electron transport chain
Chloroplasts

Keywords

  • Astaxanthin
  • high irradiance
  • lipidome
  • photooxidative stress
  • transcriptome
  • triacylglycerol

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Medicine(all)

Cite this

Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis. / Gwak, Yunho; Hwang, Yong Sic; Wang, Baobei; Kim, Minju; Jeong, Jooyeon; Lee, Choul Gyun; Hu, Qiang; Han, Danxiang; Jin, Eonseon.

In: Journal of Experimental Botany, Vol. 65, No. 15, 2014, p. 4317-4334.

Research output: Contribution to journalArticle

Gwak, Yunho ; Hwang, Yong Sic ; Wang, Baobei ; Kim, Minju ; Jeong, Jooyeon ; Lee, Choul Gyun ; Hu, Qiang ; Han, Danxiang ; Jin, Eonseon. / Comparative analyses of lipidomes and transcriptomes reveal a concerted action of multiple defensive systems against photooxidative stress in Haematococcus pluvialis. In: Journal of Experimental Botany. 2014 ; Vol. 65, No. 15. pp. 4317-4334.
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