Compact microscope-based optical tweezers system for molecular manipulation

Andy Sischka, Rainer Eckel, Katja Toensing, Robert Ros, Dario Anselmetti

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A compact single beam optical tweezers system for force measurements and manipulation of individual double-stranded deoxyribonucleic acid (DNA) molecules was discussed. The system was based on a commercial inverted optical microscope. The force response of individual DNA molecules in the presence of DNA binding agents was investigated by using an integrated liquid handling system with fluid cell. It was observed that the comparison of YOYO-1-, ethidium bromide intercalated DNA and distamycin-A complexed DNA revealed accurate and reproducible differences in the force response to an external load.

Original languageEnglish (US)
Pages (from-to)4827-4831
Number of pages5
JournalReview of Scientific Instruments
Volume74
Issue number11
DOIs
StatePublished - Nov 1 2003

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ASJC Scopus subject areas

  • Instrumentation

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