A compact single beam optical tweezers system for force measurements and manipulation of individual double-stranded deoxyribonucleic acid (DNA) molecules was discussed. The system was based on a commercial inverted optical microscope. The force response of individual DNA molecules in the presence of DNA binding agents was investigated by using an integrated liquid handling system with fluid cell. It was observed that the comparison of YOYO-1-, ethidium bromide intercalated DNA and distamycin-A complexed DNA revealed accurate and reproducible differences in the force response to an external load.
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