Community structure and function in a H2-based membrane biofilm reactor capable of bioreduction of selenate and chromate

Jinwook Chung, Hodon Ryu, Morteza Abbaszadegan, Bruce Rittmann

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Two different H2-based, denitrifying membrane-biofilm reactors (MBfRs) initially reduced Se(VI) or Cr(VI) stably to Se0 or Cr(III). When the oxidized contaminants in the influent were switched, each new oxidized contaminant was reduced immediately, and its reduction soon was approximately the same or greater than it had been in its original MBfR. The precipitation of reduced selenium and chromium in the biofilm was verified by scanning electron microscopy and energy dispersive X-ray analysis. These results on selenate and chromate reduction are consistent with the interpretation that the H 2-based biofilm community had a high level of functional diversity. The communities' structures were assessed by cloning analysis. Dechloromonas spp., a known perchlorate-reducing bacteria, dominated the clones from both reactors during selenate and chromate reductions, which suggests that it may have functional diversity capable of reducing selenate and chromate as secondary and dissimilatory acceptors.

Original languageEnglish (US)
Pages (from-to)1330-1339
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume72
Issue number6
DOIs
StatePublished - Oct 2006

Fingerprint

Selenic Acid
Chromates
Biofilms
Membranes
Impurities
Energy dispersive X ray analysis
Cloning
Selenium
Chromium
Electron Scanning Microscopy
Organism Cloning
Bacteria
Clone Cells
X-Rays
Scanning electron microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering
  • Microbiology (medical)

Cite this

@article{5cf3674727394935b3b208af2a4524ba,
title = "Community structure and function in a H2-based membrane biofilm reactor capable of bioreduction of selenate and chromate",
abstract = "Two different H2-based, denitrifying membrane-biofilm reactors (MBfRs) initially reduced Se(VI) or Cr(VI) stably to Se0 or Cr(III). When the oxidized contaminants in the influent were switched, each new oxidized contaminant was reduced immediately, and its reduction soon was approximately the same or greater than it had been in its original MBfR. The precipitation of reduced selenium and chromium in the biofilm was verified by scanning electron microscopy and energy dispersive X-ray analysis. These results on selenate and chromate reduction are consistent with the interpretation that the H 2-based biofilm community had a high level of functional diversity. The communities' structures were assessed by cloning analysis. Dechloromonas spp., a known perchlorate-reducing bacteria, dominated the clones from both reactors during selenate and chromate reductions, which suggests that it may have functional diversity capable of reducing selenate and chromate as secondary and dissimilatory acceptors.",
author = "Jinwook Chung and Hodon Ryu and Morteza Abbaszadegan and Bruce Rittmann",
year = "2006",
month = "10",
doi = "10.1007/s00253-006-0439-x",
language = "English (US)",
volume = "72",
pages = "1330--1339",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Community structure and function in a H2-based membrane biofilm reactor capable of bioreduction of selenate and chromate

AU - Chung, Jinwook

AU - Ryu, Hodon

AU - Abbaszadegan, Morteza

AU - Rittmann, Bruce

PY - 2006/10

Y1 - 2006/10

N2 - Two different H2-based, denitrifying membrane-biofilm reactors (MBfRs) initially reduced Se(VI) or Cr(VI) stably to Se0 or Cr(III). When the oxidized contaminants in the influent were switched, each new oxidized contaminant was reduced immediately, and its reduction soon was approximately the same or greater than it had been in its original MBfR. The precipitation of reduced selenium and chromium in the biofilm was verified by scanning electron microscopy and energy dispersive X-ray analysis. These results on selenate and chromate reduction are consistent with the interpretation that the H 2-based biofilm community had a high level of functional diversity. The communities' structures were assessed by cloning analysis. Dechloromonas spp., a known perchlorate-reducing bacteria, dominated the clones from both reactors during selenate and chromate reductions, which suggests that it may have functional diversity capable of reducing selenate and chromate as secondary and dissimilatory acceptors.

AB - Two different H2-based, denitrifying membrane-biofilm reactors (MBfRs) initially reduced Se(VI) or Cr(VI) stably to Se0 or Cr(III). When the oxidized contaminants in the influent were switched, each new oxidized contaminant was reduced immediately, and its reduction soon was approximately the same or greater than it had been in its original MBfR. The precipitation of reduced selenium and chromium in the biofilm was verified by scanning electron microscopy and energy dispersive X-ray analysis. These results on selenate and chromate reduction are consistent with the interpretation that the H 2-based biofilm community had a high level of functional diversity. The communities' structures were assessed by cloning analysis. Dechloromonas spp., a known perchlorate-reducing bacteria, dominated the clones from both reactors during selenate and chromate reductions, which suggests that it may have functional diversity capable of reducing selenate and chromate as secondary and dissimilatory acceptors.

UR - http://www.scopus.com/inward/record.url?scp=33749845311&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749845311&partnerID=8YFLogxK

U2 - 10.1007/s00253-006-0439-x

DO - 10.1007/s00253-006-0439-x

M3 - Article

C2 - 16673108

AN - SCOPUS:33749845311

VL - 72

SP - 1330

EP - 1339

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 6

ER -